Table 2.

Embryonic lethality of ATM D2899A (G1 founder line) mice is not rescued by disruption of Nbs1, Ku80, or 53bp1

Genotype AtmTgD2899AAtm+/+ Atm+/+ AtmTgD2899AAtm+/− Atm+/− AtmTgD2899AAtm−/− Atm−/− 
Nbs1+/+ observed 10 14 10 11 
Nbs1+/+ expected 15 15 
Nbs1ΔB/+ observed 16 20 42 38 16 
Nbs1ΔB/+ expected 15 15 30 30 15 15 
Nbs1ΔB/ΔB observed 12 14 19 
Nbs1ΔB/ΔB expected 15 15 
Ku80+/+ observed 16 21 16 
Ku80+/+ expected 10 19 10 
Ku80+/− observed 21 16 41 26 
Ku80+/− expected 19 39 13 19 
Ku80−/− observed 17 
Ku80−/− expected 10 19 10 
53bp1−/− observed 14 11 52 19 
53bp1−/− expected 19 38 13 19 
Genotype AtmTgD2899AAtm+/+ Atm+/+ AtmTgD2899AAtm+/− Atm+/− AtmTgD2899AAtm−/− Atm−/− 
Nbs1+/+ observed 10 14 10 11 
Nbs1+/+ expected 15 15 
Nbs1ΔB/+ observed 16 20 42 38 16 
Nbs1ΔB/+ expected 15 15 30 30 15 15 
Nbs1ΔB/ΔB observed 12 14 19 
Nbs1ΔB/ΔB expected 15 15 
Ku80+/+ observed 16 21 16 
Ku80+/+ expected 10 19 10 
Ku80+/− observed 21 16 41 26 
Ku80+/− expected 19 39 13 19 
Ku80−/− observed 17 
Ku80−/− expected 10 19 10 
53bp1−/− observed 14 11 52 19 
53bp1−/− expected 19 38 13 19 

Shown are the expected and observed genotypes of 3-wk-old pups from breeding Nbs1ΔB/+AtmTgD2899AAtm+/− with Nbs1ΔB/+Atm+/− mice (n = 238), intercrossing Ku80+/−AtmTgD2899AAtm+/− mice (n = 207), or intercrossing 53bp1−/−AtmTgD2899AAtm+/− mice (n = 102). Note the numbers of ATM kinase-inactive live-born pups in the different Ku80 backgrounds, the comparison between the number of ATM kinase-inactive and Atm-null live-born pups in the 53bp1-/- background, and the numbers of ATM kinase-inactive live-born pups in the different Nbs1 backgrounds.

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