Summary of Delta values
| Epitope | To 9G3 | To CENP-I | Average to CENP-I |
| nm | nm | nm | |
| Zwint1 (normal) | 51 ± 4 | 13 ± 9 | 15 ± 9 |
| Zwint1 (motif mutant) | 49 ± 8 | 16 ± 11 | |
| Zwint1-C (normal) | 48 ± 4 | 17 ± 9 | 19 ± 10 |
| Zwint1-C–GFP (normal) | 44 ± 6 | 21 ± 10 | |
| Zwilch (normal) | 6 ± 11 | 59 ± 14 | 64 ± 15 |
| Zwilch (motif mutant) | −3 ± 13 | 68 ± 15 | |
| Zw10-C (motif mutant) | 29 ± 15 | 36 ± 17 | 36 ± 17 |
| GFP–Zw10-N (normal) | 13 ± 14 | 52 ± 16 | 52 ± 16 |
| Rod-N (motif mutant) | 10 ± 14 | 55 ± 16 | 55 ± 16 |
| GFP–Spindly-N (Motif mutant) | −74 ± 14 | 140 ± 17 | 140 ± 17 |
| 9G3 (motif mutant) | NA | 65 ± 8 | 65 ± 8 |
| Mad1 (motif mutant) | NA | 34 ± 15 | 34 ± 15 |
| Spindly-C (motif mutant) | −4 ± 25 | 69 ± 25 | 69 ± 25 |
| Hec1-C–tdTomato (normal) | 40 ± 5 | 25 ± 9 | 25 ± 9 |
| Epitope | To 9G3 | To CENP-I | Average to CENP-I |
| nm | nm | nm | |
| Zwint1 (normal) | 51 ± 4 | 13 ± 9 | 15 ± 9 |
| Zwint1 (motif mutant) | 49 ± 8 | 16 ± 11 | |
| Zwint1-C (normal) | 48 ± 4 | 17 ± 9 | 19 ± 10 |
| Zwint1-C–GFP (normal) | 44 ± 6 | 21 ± 10 | |
| Zwilch (normal) | 6 ± 11 | 59 ± 14 | 64 ± 15 |
| Zwilch (motif mutant) | −3 ± 13 | 68 ± 15 | |
| Zw10-C (motif mutant) | 29 ± 15 | 36 ± 17 | 36 ± 17 |
| GFP–Zw10-N (normal) | 13 ± 14 | 52 ± 16 | 52 ± 16 |
| Rod-N (motif mutant) | 10 ± 14 | 55 ± 16 | 55 ± 16 |
| GFP–Spindly-N (Motif mutant) | −74 ± 14 | 140 ± 17 | 140 ± 17 |
| 9G3 (motif mutant) | NA | 65 ± 8 | 65 ± 8 |
| Mad1 (motif mutant) | NA | 34 ± 15 | 34 ± 15 |
| Spindly-C (motif mutant) | −4 ± 25 | 69 ± 25 | 69 ± 25 |
| Hec1-C–tdTomato (normal) | 40 ± 5 | 25 ± 9 | 25 ± 9 |
Summary of Delta values obtained from measurements of 13 different epitopes spanning the Zwint1–RZZ complex, Mad1, and Spindly made relative to either the Hec1 CH (head) domain or the constitutive centromere-associated network protein CENP-I. The average distance of an epitope to CENP-I in the rightmost column refers to the average of the measurements between normal and Spindly motif metaphase kinetochores. NA, not applicable.