Bacterial strains used
| Strain | Plasmid | Host Strain |
| NRB1462 | pAT110-snc2-R75A,R79A,K82A(M1); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1463 | pAT110-snc2-R75E,R79E,K82E(M2); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1464 | pAT110-snc2-E60A,D61A,D64A(M3); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1465 | pAT110-snc2-E60K,D61R,D64R(M4); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1466 | pAT110-snc2-E51A,E54A(M5); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1467 | pAT110-snc2-E51R,E54R(M6); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1468 | pAT110-snc2-R75A; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1469 | pAT110-snc2-R75E; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1470 | pAT110-snc2-R79A; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1471 | pAT110-snc2-R79E; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1472 | pAT110-snc2-K82A; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1473 | pAT110-snc2-K82E; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1474 | pQlink-His-TEV-Sec6 (1–805 aa); Ampr | Rosetta |
| NRB1475 | pQlink-His-TEV-Sec6 (292–805 aa); Ampr | Rosetta |
| NRB1476 | pQlink-His-TEV-Sec6 (411–805 aa); Ampr | Rosetta |
| NRB1477 | pQlink-His-TEV-Sec6 (1–411 aa); pCDF-GST-PreScission-Sec8(1–236 aa); Strepr,Ampr | Rosetta |
| NRB1478 | pQlink-His-TEV-Sec6 (1–292 aa); pCDF-GST-PreScission-Sec8(1–236 aa); Strepr,Ampr | Rosetta |
| NRB1480 | pCDF-GST-PreScission-Sec6(100–805 aa); Strepr | Rosetta |
| NRB1481 | pAT110-SNC2 (1–92 aa); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1482 | pAT110-SNC2 (28–92 aa); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1483 | pAT110-SNC2 (1–52 aa); inserted into BamHI–EcoRI sites of pAT110;kanr | Rosetta |
| NRB1484 | pAT110-SNC2 (53–92 aa); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1485 | pET46-Sec6;Ampr | Rosetta |
| NRB1486 | pSV282-Sec9CT (416–651 aa); inserted into BamHI–EcoRI sites of pSV282;kanr | Rosetta |
| NRB1487 | pAT110;kanr | Rosetta |
| NRB1488 | pAT110-Vamp3; human Vamp3 cDNA inserted into BamH1–EcoR1 sites of pAT110 kanr | Rosetta |
| NRB1489 | pAT110-Vamp4; human Vamp4 cDNA inserted into the BamH1–Xho1 sites of pAT110 kanr | Rosetta |
| Strain | Plasmid | Host Strain |
| NRB1462 | pAT110-snc2-R75A,R79A,K82A(M1); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1463 | pAT110-snc2-R75E,R79E,K82E(M2); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1464 | pAT110-snc2-E60A,D61A,D64A(M3); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1465 | pAT110-snc2-E60K,D61R,D64R(M4); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1466 | pAT110-snc2-E51A,E54A(M5); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1467 | pAT110-snc2-E51R,E54R(M6); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1468 | pAT110-snc2-R75A; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1469 | pAT110-snc2-R75E; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1470 | pAT110-snc2-R79A; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1471 | pAT110-snc2-R79E; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1472 | pAT110-snc2-K82A; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1473 | pAT110-snc2-K82E; inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1474 | pQlink-His-TEV-Sec6 (1–805 aa); Ampr | Rosetta |
| NRB1475 | pQlink-His-TEV-Sec6 (292–805 aa); Ampr | Rosetta |
| NRB1476 | pQlink-His-TEV-Sec6 (411–805 aa); Ampr | Rosetta |
| NRB1477 | pQlink-His-TEV-Sec6 (1–411 aa); pCDF-GST-PreScission-Sec8(1–236 aa); Strepr,Ampr | Rosetta |
| NRB1478 | pQlink-His-TEV-Sec6 (1–292 aa); pCDF-GST-PreScission-Sec8(1–236 aa); Strepr,Ampr | Rosetta |
| NRB1480 | pCDF-GST-PreScission-Sec6(100–805 aa); Strepr | Rosetta |
| NRB1481 | pAT110-SNC2 (1–92 aa); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1482 | pAT110-SNC2 (28–92 aa); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1483 | pAT110-SNC2 (1–52 aa); inserted into BamHI–EcoRI sites of pAT110;kanr | Rosetta |
| NRB1484 | pAT110-SNC2 (53–92 aa); inserted into BamHI–XhoI sites of pAT110;kanr | Rosetta |
| NRB1485 | pET46-Sec6;Ampr | Rosetta |
| NRB1486 | pSV282-Sec9CT (416–651 aa); inserted into BamHI–EcoRI sites of pSV282;kanr | Rosetta |
| NRB1487 | pAT110;kanr | Rosetta |
| NRB1488 | pAT110-Vamp3; human Vamp3 cDNA inserted into BamH1–EcoR1 sites of pAT110 kanr | Rosetta |
| NRB1489 | pAT110-Vamp4; human Vamp4 cDNA inserted into the BamH1–Xho1 sites of pAT110 kanr | Rosetta |