Table 4.

Plasmids for yeast transformation or HeLa cell transfection

Name Plasmid 
pNB300 YCp50, GAL1-Sec15 
PNB882 pRS306 SEC6-GFP 
pNB1304 pRS306 SEC5-3xGFP 
pNB1308 pRS306 SEC15-3xGFP 
pNB1456 YCp50 GAL1-SEC15-GFP; a fragment of Sec15-GFP into BamH I–SalI sites of pNB300 
pNB1457 pRS425 Sec4, a 392-bp Sec4 fragment was ligated into BamHI–XhoI sites 
pNB1458 pRS303 Sec15-3xGFP; inserting Sec15-3xGFP-ADHterm fragment into pRS303 SalI–SacI sites 
pNB1459 pRS305 Sec6-2xmCherry; inserted 1.6 kB Sec6 C-terminal fragment into XhoI–XbaI sites of pJC1-2xmCherry 
pNB1460 pRS305 Sec8-2xmCherry; inserted 1 kB Sec8 C-terminal fragment into XhoI–XbaI sites of pJC1-2xmCherry 
pNB1461 pRS306 Snc2-M2(75E79E82E) 
pNB 1462 pCMV-myc-Exoc3 
pSFNB1223 pRS416 GFP-SNC1 
Name Plasmid 
pNB300 YCp50, GAL1-Sec15 
PNB882 pRS306 SEC6-GFP 
pNB1304 pRS306 SEC5-3xGFP 
pNB1308 pRS306 SEC15-3xGFP 
pNB1456 YCp50 GAL1-SEC15-GFP; a fragment of Sec15-GFP into BamH I–SalI sites of pNB300 
pNB1457 pRS425 Sec4, a 392-bp Sec4 fragment was ligated into BamHI–XhoI sites 
pNB1458 pRS303 Sec15-3xGFP; inserting Sec15-3xGFP-ADHterm fragment into pRS303 SalI–SacI sites 
pNB1459 pRS305 Sec6-2xmCherry; inserted 1.6 kB Sec6 C-terminal fragment into XhoI–XbaI sites of pJC1-2xmCherry 
pNB1460 pRS305 Sec8-2xmCherry; inserted 1 kB Sec8 C-terminal fragment into XhoI–XbaI sites of pJC1-2xmCherry 
pNB1461 pRS306 Snc2-M2(75E79E82E) 
pNB 1462 pCMV-myc-Exoc3 
pSFNB1223 pRS416 GFP-SNC1 

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