Quantification of phenotypes
| Conditions | Embryonic lethality | Position at PN meeting | Spindle misorientation |
| % | |||
| Wild type | 0 (n = 217) | 68.3 ± 0.7 (n = 11) | 0 (n = 11) |
| ubxn-2(RNAi) | 82.73 ± 4.97 (n = 274) | 74.7 ± 1.4e (n = 33) | 39.1 (n = 19) |
| GFP::UBXN-2R | 0 (n = 279) | ND | 0 (n = 16) |
| GFP::UBXN-2R ubxn-2(RNAi) | 0 (n = 256) | 66.4 ± 0.1 (n = 13) | 0 (n = 10) |
| ubxn-2(ok1942)/+ a | 51 ± 4 (n = 491) | 67.7 ± 1.1 (n = 12) | 0 (n = 9) |
| +/+ (VC362)b | 47.7 ± 14.1 (n = 312) | 67.4 ± 0.1 (n = 10) | 0 (n = 10) |
| ubxn-2(ok1942)/+ ubxn-2(RNAi) | 81.7 ± 10.4 (n = 348) | 77 ± 1.4d (n = 36) | 72c (n = 155) |
| Conditions | Embryonic lethality | Position at PN meeting | Spindle misorientation |
| % | |||
| Wild type | 0 (n = 217) | 68.3 ± 0.7 (n = 11) | 0 (n = 11) |
| ubxn-2(RNAi) | 82.73 ± 4.97 (n = 274) | 74.7 ± 1.4e (n = 33) | 39.1 (n = 19) |
| GFP::UBXN-2R | 0 (n = 279) | ND | 0 (n = 16) |
| GFP::UBXN-2R ubxn-2(RNAi) | 0 (n = 256) | 66.4 ± 0.1 (n = 13) | 0 (n = 10) |
| ubxn-2(ok1942)/+ a | 51 ± 4 (n = 491) | 67.7 ± 1.1 (n = 12) | 0 (n = 9) |
| +/+ (VC362)b | 47.7 ± 14.1 (n = 312) | 67.4 ± 0.1 (n = 10) | 0 (n = 10) |
| ubxn-2(ok1942)/+ ubxn-2(RNAi) | 81.7 ± 10.4 (n = 348) | 77 ± 1.4d (n = 36) | 72c (n = 155) |
Lethality is expressed as mean ± SEM. Pronuclear position at meeting is calculated as percentage of embryo length ± SEM.
VC1450 strain. n = numbers of embryos analyzed.
VC362 expresses the balancer used to balance ubxn-2(ok1942). The one-cell embryos behaved as wild-type embryos.
The spindle orientation defects had a variable penetrance between experiments (50–90%).
P < 0.01 compared to wild-type N2.
P < 0.05 for the difference between wild-type and RNAi-treated embryos (t test).