Table I. Summary of the effect of... | Rockefeller University Press

Table I.

Summary of the effect of different gain and loss-of-function constructs on LGN and NuMA distribution, spindle orientation, and production of ectopic neural progenitors

Condition	LGN distribution^b	NuMA distribution	Spindle orientation	Ectopic progenitors at E4^c
Wild type	Lateral cortical ring	Cortical ring/spindle poles	Planar	−
LGN RNAi	−	No ring/spindle poles	Random	++^a
Ct-LGN	Cytoplasmic^a	No ring/spindle poles	Random^a	++^a
LGN RNAi + mLGN rescue	ND	ND	Planar (wild type)^a	−^a
NuMA RNAi	Cortical	−	Defects	+
Gαi1/2 RNAi	ND	ND	Defects	+
PTx-A	ND	ND	Defects	++
ratGαi1-G203A	Strong cortical	No ring/faint cortical?/spindle poles	Random	++
ratGαi1	Strong cortical	ND	ND	++
Cyto-ratGαi1	ND	ND	ND	++
ratGαi2	ND	ND	ND	++
ratGαi3	ND	ND	ND	++
Human Gαtransducin	ND	ND	ND	++
ratGαolf	ND	ND	ND	−
ratGαolf-G213A	Cortical (wild type)	ND	Planar (wild type)	−

Condition	LGN distribution^b	NuMA distribution	Spindle orientation	Ectopic progenitors at E4^c
Wild type	Lateral cortical ring	Cortical ring/spindle poles	Planar	−
LGN RNAi	−	No ring/spindle poles	Random	++^a
Ct-LGN	Cytoplasmic^a	No ring/spindle poles	Random^a	++^a
LGN RNAi + mLGN rescue	ND	ND	Planar (wild type)^a	−^a
NuMA RNAi	Cortical	−	Defects	+
Gαi1/2 RNAi	ND	ND	Defects	+
PTx-A	ND	ND	Defects	++
ratGαi1-G203A	Strong cortical	No ring/faint cortical?/spindle poles	Random	++
ratGαi1	Strong cortical	ND	ND	++
Cyto-ratGαi1	ND	ND	ND	++
ratGαi2	ND	ND	ND	++
ratGαi3	ND	ND	ND	++
Human Gαtransducin	ND	ND	ND	++
ratGαolf	ND	ND	ND	−
ratGαolf-G213A	Cortical (wild type)	ND	Planar (wild type)	−

ND, not determined.

Morin et al, 2007.

Data from overexpression of GFP-tagged chick LGN or Myc-tagged mouse LGN.

Ectopic progenitors are determined by the presence of BrdU+ cells in the mantle zone on transverse sections at E4 after a 45-min BrdU pulse (see Morin et al, 2007).

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