Genetic interactions of epg-7 with other autophagy mutants
| Genotype | Percentage of SQST-1 aggregates forming clusters (n)a | Percentage of LGG-1 puncta forming clusters (n)a | Percentage of LGG-1 puncta colocalizing with SQST-1 aggregates (n)ab |
| wild type | NAd | 2.3 (389) | NA |
| epg-7 | 3.4 (498) | 2.2 (311) | 13.9 (311) |
| epg-6 | 36.1 (368) | 24.5 (290) | 91.5 (290) |
| epg-6;epg-7 | 5.0 (382) | 3.1 (225) | 19.4 (225) |
| epg-3 | 26.0 (373) | 23.0 (280) | 91.6 (280) |
| epg-3;epg-7 | 5.3 (415) | 5.9 (273) | 6.9 (273) |
| epg-4 | 27.4 (415) | 24.4 (290) | 91.9 (290) |
| epg-4;epg-7 | 5.5 (367) | 3.1(256) | 14.6 (256) |
| atg-9c | 7.5 (477) | 4.7 (303) | 82.4 (303) |
| atg-9;epg-7 | 14.3 (343) | 0 (32) | 10.2 (32) |
| atg-18 | 13.6 (315) | 9.7 (259) | 87.7 (259) |
| atg-18;epg-7 | 9.4 (436) | 3.7 (326) | 6.2 (326) |
| Genotype | Percentage of SQST-1 aggregates forming clusters (n)a | Percentage of LGG-1 puncta forming clusters (n)a | Percentage of LGG-1 puncta colocalizing with SQST-1 aggregates (n)ab |
| wild type | NAd | 2.3 (389) | NA |
| epg-7 | 3.4 (498) | 2.2 (311) | 13.9 (311) |
| epg-6 | 36.1 (368) | 24.5 (290) | 91.5 (290) |
| epg-6;epg-7 | 5.0 (382) | 3.1 (225) | 19.4 (225) |
| epg-3 | 26.0 (373) | 23.0 (280) | 91.6 (280) |
| epg-3;epg-7 | 5.3 (415) | 5.9 (273) | 6.9 (273) |
| epg-4 | 27.4 (415) | 24.4 (290) | 91.9 (290) |
| epg-4;epg-7 | 5.5 (367) | 3.1(256) | 14.6 (256) |
| atg-9c | 7.5 (477) | 4.7 (303) | 82.4 (303) |
| atg-9;epg-7 | 14.3 (343) | 0 (32) | 10.2 (32) |
| atg-18 | 13.6 (315) | 9.7 (259) | 87.7 (259) |
| atg-18;epg-7 | 9.4 (436) | 3.7 (326) | 6.2 (326) |
Confocal images of embryos at the comma stage were determined for atg-9 and atg-9;epg-7 mutants. ∼200-cell stage embryos were examined for other autophagy mutants. A cluster is defined as two or more punctate structures interconnected.
Colocalization refers to two aggregates showing >70% overlap in their fluorescence signals.
LGG-1 accumulates into large aggregates in some cells in atg-9 mutant. Those aggregates are not classified as clusters.
NA: SQST-1 aggregates are absent in wild-type embryos.