Quantification of Western blot analyses
| Antibody target | P7 brain | Adult brain | ||
| Control | RibKO | Control | RibKO | |
| rictor | 100 ± 8.1 | 13.7 ± 2.4*** | 100 ± 24 | n.d. |
| mTOR | 100 ± 10.5 | 108.5 ± 7.9 | 100 ± 31 | 64.9 ± 7.5 |
| P-mTOR (Ser2481) | 100 ± 2.8 | 21.2 ± 5*** | 100 ± 25.2 | 2.7 ± 2.2** |
| P-mTOR (Ser2448) | 100 ± 7 | 96.5 ± 12.3 | 100 ± 20.7 | 79 ± 21 |
| S6K | 100 ± 12.2 | 93.6 ± 8.2 | 100 ± 8.8 | 91.4 ± 5.8 |
| PS6K (Thr389) | 100 ± 8.3 | 116.5 ± 5.9 | 100 ± 5.2 | 85.6± 9.2 |
| S6 | 100 ± 9.1 | 107.2 ± 13.8 | 100 ± 6.1 | 169.3 ± 29.4 |
| P-S6 (Ser235/236) | 100 ± 6.2 | 83.2 ± 3.3 | 100 ± 10 | 105.2 ± 17.5 |
| P-4E-BP (Thr37/46) | 100 ± 8.3 | 94 ± 7.6 | 100 ± 11 | 179.7 ± 37.2 |
| P-eIF4E (Ser209) | 100 ± 13 | 84.6 ± 3.1 | 100 ± 10 | 107.0 ± 13.8 |
| P-TSC2 (Thr1462) | 100 ± 9.8 | 177.5 ± 45.6 | 100 ± 22 | 80.2 ± 22.4 |
| Akt | 100 ± 10.5 | 127.6 ± 17.3 | 100 ± 4.7 | 99.4 ± 6.1 |
| P-Akt (Ser473) | 100 ± 10 | 3.9 ± 0.9*** | 100 ± 12 | 18.3 ± 4.8*** |
| P-Akt (Thr308) | 100 ± 7 | 52 ± 17 | 100 ± 15 | 36.9 ± 8.1** |
| P-FoxO1 (Ser256) | 100 ± 15.7 | 141.1 ± 13.3 | 100 ± 9.3 | 164.6 ± 26.7 |
| GSK3-β | 100 ± 5.0 | 101.5 ± 5.5 | 100 ± 5.1 | 97.1 ± 4.7 |
| P-GSK3-β (Ser9) | 100 ± 11.7 | 115.8 ± 12.4 | 100 ± 17 | 103.7 ± 5.1 |
| NDRG1 | 100 ± 19.8 | 115.3 ± 6.5 | 100 ± 5.4 | 94.2 ± 7.9 |
| P-NDRG1 (T346) | 100 ± 13.7 | 92 ± 15.9 | 100 ± 3.8 | 52.4 ± 7.3*** |
| PKCα | 100 ± 6.8 | 55.8 ± 3.4*** | 100 ± 23.8 | 2.6 ± 1.7*** |
| PKCβ2 | 100 ± 7.0 | 78.6 ± 3.7* | 100 ± 11.4 | 3.7 ± 0.4*** |
| PKCγ | 100 ± 6.2 | 58.5 ± 2.1*** | 100 ± 6.6 | 7.4 ± 1.8*** |
| PKCε | 100 ± 12 | 17.7 ± 2.8*** | 100 ± 22.9 | 15.5 ± 5* |
| PKCζ | 100 ± 17.1 | 106 ± 11.9 | 100 ± 14.6 | 60.0 ± 7.8 |
| GAP-43 | 100 ± 1.2 | 105 ± 5.8 | 100 ± 5 | 99.9 ± 6.6 |
| P-GAP-43 (Ser41) | 100 ± 13.1 | 22.4 ± 3.9** | 100 ± 27 | 19.5 ± 4.7** |
| MARCKS | 100 ± 2.3 | 94.2 ± 3.4 | 100 ± 22 | 109.7 ± 3 |
| P-MARCKS | 100 ± 8.8 | n.d. | 100 ± 25 | n.d. |
| Antibody target | P7 brain | Adult brain | ||
| Control | RibKO | Control | RibKO | |
| rictor | 100 ± 8.1 | 13.7 ± 2.4*** | 100 ± 24 | n.d. |
| mTOR | 100 ± 10.5 | 108.5 ± 7.9 | 100 ± 31 | 64.9 ± 7.5 |
| P-mTOR (Ser2481) | 100 ± 2.8 | 21.2 ± 5*** | 100 ± 25.2 | 2.7 ± 2.2** |
| P-mTOR (Ser2448) | 100 ± 7 | 96.5 ± 12.3 | 100 ± 20.7 | 79 ± 21 |
| S6K | 100 ± 12.2 | 93.6 ± 8.2 | 100 ± 8.8 | 91.4 ± 5.8 |
| PS6K (Thr389) | 100 ± 8.3 | 116.5 ± 5.9 | 100 ± 5.2 | 85.6± 9.2 |
| S6 | 100 ± 9.1 | 107.2 ± 13.8 | 100 ± 6.1 | 169.3 ± 29.4 |
| P-S6 (Ser235/236) | 100 ± 6.2 | 83.2 ± 3.3 | 100 ± 10 | 105.2 ± 17.5 |
| P-4E-BP (Thr37/46) | 100 ± 8.3 | 94 ± 7.6 | 100 ± 11 | 179.7 ± 37.2 |
| P-eIF4E (Ser209) | 100 ± 13 | 84.6 ± 3.1 | 100 ± 10 | 107.0 ± 13.8 |
| P-TSC2 (Thr1462) | 100 ± 9.8 | 177.5 ± 45.6 | 100 ± 22 | 80.2 ± 22.4 |
| Akt | 100 ± 10.5 | 127.6 ± 17.3 | 100 ± 4.7 | 99.4 ± 6.1 |
| P-Akt (Ser473) | 100 ± 10 | 3.9 ± 0.9*** | 100 ± 12 | 18.3 ± 4.8*** |
| P-Akt (Thr308) | 100 ± 7 | 52 ± 17 | 100 ± 15 | 36.9 ± 8.1** |
| P-FoxO1 (Ser256) | 100 ± 15.7 | 141.1 ± 13.3 | 100 ± 9.3 | 164.6 ± 26.7 |
| GSK3-β | 100 ± 5.0 | 101.5 ± 5.5 | 100 ± 5.1 | 97.1 ± 4.7 |
| P-GSK3-β (Ser9) | 100 ± 11.7 | 115.8 ± 12.4 | 100 ± 17 | 103.7 ± 5.1 |
| NDRG1 | 100 ± 19.8 | 115.3 ± 6.5 | 100 ± 5.4 | 94.2 ± 7.9 |
| P-NDRG1 (T346) | 100 ± 13.7 | 92 ± 15.9 | 100 ± 3.8 | 52.4 ± 7.3*** |
| PKCα | 100 ± 6.8 | 55.8 ± 3.4*** | 100 ± 23.8 | 2.6 ± 1.7*** |
| PKCβ2 | 100 ± 7.0 | 78.6 ± 3.7* | 100 ± 11.4 | 3.7 ± 0.4*** |
| PKCγ | 100 ± 6.2 | 58.5 ± 2.1*** | 100 ± 6.6 | 7.4 ± 1.8*** |
| PKCε | 100 ± 12 | 17.7 ± 2.8*** | 100 ± 22.9 | 15.5 ± 5* |
| PKCζ | 100 ± 17.1 | 106 ± 11.9 | 100 ± 14.6 | 60.0 ± 7.8 |
| GAP-43 | 100 ± 1.2 | 105 ± 5.8 | 100 ± 5 | 99.9 ± 6.6 |
| P-GAP-43 (Ser41) | 100 ± 13.1 | 22.4 ± 3.9** | 100 ± 27 | 19.5 ± 4.7** |
| MARCKS | 100 ± 2.3 | 94.2 ± 3.4 | 100 ± 22 | 109.7 ± 3 |
| P-MARCKS | 100 ± 8.8 | n.d. | 100 ± 25 | n.d. |
Quantification of protein and phosphorylation levels of proteins shown in Fig. 7. Percentage given represents the relative intensity of the protein bands as detected by Western blot analysis from control and RibKO mice. Equal amount of protein was loaded and immunodetection of β-tubulin, GAPDH, β-actin, or pan-actin served as loading control. Amount of each protein listed was normalized to loading control. All values obtained in controls were set to 100%. Data represent mean ± SEM from n ≥ 3–15 mice for each genotype. n.d., not detected. Student’s t test: ***, P < 0.001; **, P < 0.01; *, P < 0.05.