Yeast strains used in this study
| Name | Genotype |
| DMY1735 | MATα, CDC28::Neo, ABP1-RFP::HIS3, GFP-SEC4::URA3, his3Δ200, lys2-801, ura3-52, leu2-3,112 |
| DMY1731 | MATα, CDC28::Neo, Δsla2::LEU2, ABP1-RFP::HIS3, GFP-SEC4::URA3, his3Δ200, lys2-801, ura3-52, leu2-3,112 |
| DMY1738 | MATα, CDC28::NEO1, ABP1-RFP::HIS3,RVS167-GFP:: kanMX6, his3Δ200, lys2-801, ura3-52, leu2-3,112 |
| DMY1804 | MATa, SSD1-V, Δrvs161:: kanMX6, Δrvs167::natNT2, ABP1-RFP::HIS3, his3^1, leu2^0 met15^0, ura3^0 |
| DMY1809 | MATa, SSD1-V, Δsla1:: kanMX6, Δbbc1:: natNT2, ABP1-RFP::HIS3, his3^1, leu2^0 met15^0, ura3^0 |
| DMY1851 | MATa, SSD1-V, Δede1:: kanMX6, ABP1-RFP::HIS3, GFP-SEC4::URA3, his3^1, leu2^0 met15^0, ura3^0 |
| DMY1865 | MATa, SSD1-V, Δclc1:: kanMX6, ABP1-RFP::HIS3, GFP-SEC4::URA3, his3^1, leu2^0 met15^0, ura3^0 |
| DMY1853 | MATα, CDC28::Neo, Δsla2::LEU2, ABP1-RFP::HIS3, RVS167-GFP:: kanMX6, his3Δ200, lys2-801, ura3-52, leu2-3,112 |
| Name | Genotype |
| DMY1735 | MATα, CDC28::Neo, ABP1-RFP::HIS3, GFP-SEC4::URA3, his3Δ200, lys2-801, ura3-52, leu2-3,112 |
| DMY1731 | MATα, CDC28::Neo, Δsla2::LEU2, ABP1-RFP::HIS3, GFP-SEC4::URA3, his3Δ200, lys2-801, ura3-52, leu2-3,112 |
| DMY1738 | MATα, CDC28::NEO1, ABP1-RFP::HIS3,RVS167-GFP:: kanMX6, his3Δ200, lys2-801, ura3-52, leu2-3,112 |
| DMY1804 | MATa, SSD1-V, Δrvs161:: kanMX6, Δrvs167::natNT2, ABP1-RFP::HIS3, his3^1, leu2^0 met15^0, ura3^0 |
| DMY1809 | MATa, SSD1-V, Δsla1:: kanMX6, Δbbc1:: natNT2, ABP1-RFP::HIS3, his3^1, leu2^0 met15^0, ura3^0 |
| DMY1851 | MATa, SSD1-V, Δede1:: kanMX6, ABP1-RFP::HIS3, GFP-SEC4::URA3, his3^1, leu2^0 met15^0, ura3^0 |
| DMY1865 | MATa, SSD1-V, Δclc1:: kanMX6, ABP1-RFP::HIS3, GFP-SEC4::URA3, his3^1, leu2^0 met15^0, ura3^0 |
| DMY1853 | MATα, CDC28::Neo, Δsla2::LEU2, ABP1-RFP::HIS3, RVS167-GFP:: kanMX6, his3Δ200, lys2-801, ura3-52, leu2-3,112 |
DMY1735, 1731, 1738, and 1853 were derived from DDY904 (Drubin laboratory). The Neo marker is integrated downstream of WT CDC28 in the strains. DMY1804 and 1809 were transformed with a CEN LEU2 GFP-Sec4 plasmid (pDM18) for imaging.