Plasmids used in this study
| Name | Expressed protein | Reference |
| pcDNAZeo/HA-PEX26 | Full-length human PEX26 with an N-terminal 2×HA tag | This study |
| pcDNAZeo/FLAG-PEX26 | Full-length human PEX26 with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26ΔC | PEX26ΔC (aa 2–246) with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26ΔCS | PEX26ΔCS (aa 2–270) with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26-K1S | PEX26-K1S (Fig. 6) with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26-RRK3S | PEX26-RRK3S (Fig. 6) with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26-KRRK4S | PEX26-KRRK4S (Fig. 6) with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26-CHARGE | PEX26-CHARGE (Fig. 6) with an N-terminal FLAG tag | This study |
| pcDNAZeo/EGFP-PEX26 | Full-length human PEX26 with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-PEX26ΔCS | PEX26ΔCS with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-PEX26-K1S | PEX26-K1S with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-PEX26-RRK3S | PEX26-RRK3S with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-PEX26-KRRK4S | PEX26-KRRK4S with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-PEX26-CHARGE | PEX26-CHARGE with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-OMP25 | Full-length mouse OMP25 with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-Cyt b5 | Full-length mouse Cyt b5 with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-Sec61β | Full-length human Sec61β with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-VAMP2 | Full-length mouse VAMP2 with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP–OMP25-R | OMP25-R (Fig. 7) with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP–Cyt b5-R | Cyt b5-R (Fig. 7) with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP–Sec61β-R | Sec61β-R (Fig. 7) with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP–VAMP2-R | VAMP2-R (Fig. 7) with an N-terminal EGFP | This study |
| pcDNAZeo/FLAG-OMP25 | Full-length mouse OMP25 with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-Cyt b5 | Full-length mouse Cyt b5 with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-Sec61β | Full-length human Sec61β with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-VAMP2 | Full-length mouse VAMP2 with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG–Sec61β-R | Sec61β-R with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG–VAMP2-R | VAMP2-R with an N-terminal FLAG tag | This study |
| pcDNAZeo/Myc-Sec61 | Full-length human Sec61β with an N-terminal 6×Myc tag | This study |
| pcDNAZeo/HA-PEX19 | Full-length human PEX19 with an N-terminal 2×HA tag | Matsuzono et al., 2006 |
| pcDNAZeo/FLAG-PEX19 | Full-length human PEX19 with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX19ΔN23 | PEX19ΔN23 (aa 24–299) with an N-terminal FLAG tag | This study |
| pcDNAZeo/Myc-PEX19 | Full-length human PEX19 with an N-terminal Myc tag | This study |
| pcDNAZeo/Myc-PEX19ΔN23 | PEX19ΔN23 with an N-terminal Myc tag | This study |
| pcDNAZeo/PEX3-EGFP | Full-length rat PEX3 with a C-terminal EGFP | This study |
| pcDNAZeo/PEX3-W104A-EGFP | PEX3 carrying the W104A mutation with a C-terminal EGFP | This study |
| pcDNAZeo/Mito-PEX3-EGFP | A chimeric protein, termed Mito-PEX3-EGFP, comprising aa 1–69 of rat Tom20, aa 40–373 of rat PEX3, and EGFP | This study |
| pcDNAZeo/Mito-PEX3-W104A-EGFP | Identical to Mito-PEX3-EGFP except that PEX3 in this construct harbors the W104A mutation | This study |
| pcDNAZeo/ER-EGFP | A fusion protein comprising aa 1–414 of human Hsp47, EGFP, and a KDEL retention signal | This study |
| pEGFP-C1/EGFP-PEX16 | Full-length human PEX16 with an N-terminal EGFP | Matsuzaki and Fujiki, 2008 |
| Name | Expressed protein | Reference |
| pcDNAZeo/HA-PEX26 | Full-length human PEX26 with an N-terminal 2×HA tag | This study |
| pcDNAZeo/FLAG-PEX26 | Full-length human PEX26 with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26ΔC | PEX26ΔC (aa 2–246) with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26ΔCS | PEX26ΔCS (aa 2–270) with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26-K1S | PEX26-K1S (Fig. 6) with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26-RRK3S | PEX26-RRK3S (Fig. 6) with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26-KRRK4S | PEX26-KRRK4S (Fig. 6) with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX26-CHARGE | PEX26-CHARGE (Fig. 6) with an N-terminal FLAG tag | This study |
| pcDNAZeo/EGFP-PEX26 | Full-length human PEX26 with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-PEX26ΔCS | PEX26ΔCS with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-PEX26-K1S | PEX26-K1S with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-PEX26-RRK3S | PEX26-RRK3S with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-PEX26-KRRK4S | PEX26-KRRK4S with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-PEX26-CHARGE | PEX26-CHARGE with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-OMP25 | Full-length mouse OMP25 with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-Cyt b5 | Full-length mouse Cyt b5 with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-Sec61β | Full-length human Sec61β with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP-VAMP2 | Full-length mouse VAMP2 with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP–OMP25-R | OMP25-R (Fig. 7) with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP–Cyt b5-R | Cyt b5-R (Fig. 7) with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP–Sec61β-R | Sec61β-R (Fig. 7) with an N-terminal EGFP | This study |
| pcDNAZeo/EGFP–VAMP2-R | VAMP2-R (Fig. 7) with an N-terminal EGFP | This study |
| pcDNAZeo/FLAG-OMP25 | Full-length mouse OMP25 with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-Cyt b5 | Full-length mouse Cyt b5 with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-Sec61β | Full-length human Sec61β with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-VAMP2 | Full-length mouse VAMP2 with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG–Sec61β-R | Sec61β-R with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG–VAMP2-R | VAMP2-R with an N-terminal FLAG tag | This study |
| pcDNAZeo/Myc-Sec61 | Full-length human Sec61β with an N-terminal 6×Myc tag | This study |
| pcDNAZeo/HA-PEX19 | Full-length human PEX19 with an N-terminal 2×HA tag | Matsuzono et al., 2006 |
| pcDNAZeo/FLAG-PEX19 | Full-length human PEX19 with an N-terminal FLAG tag | This study |
| pcDNAZeo/FLAG-PEX19ΔN23 | PEX19ΔN23 (aa 24–299) with an N-terminal FLAG tag | This study |
| pcDNAZeo/Myc-PEX19 | Full-length human PEX19 with an N-terminal Myc tag | This study |
| pcDNAZeo/Myc-PEX19ΔN23 | PEX19ΔN23 with an N-terminal Myc tag | This study |
| pcDNAZeo/PEX3-EGFP | Full-length rat PEX3 with a C-terminal EGFP | This study |
| pcDNAZeo/PEX3-W104A-EGFP | PEX3 carrying the W104A mutation with a C-terminal EGFP | This study |
| pcDNAZeo/Mito-PEX3-EGFP | A chimeric protein, termed Mito-PEX3-EGFP, comprising aa 1–69 of rat Tom20, aa 40–373 of rat PEX3, and EGFP | This study |
| pcDNAZeo/Mito-PEX3-W104A-EGFP | Identical to Mito-PEX3-EGFP except that PEX3 in this construct harbors the W104A mutation | This study |
| pcDNAZeo/ER-EGFP | A fusion protein comprising aa 1–414 of human Hsp47, EGFP, and a KDEL retention signal | This study |
| pEGFP-C1/EGFP-PEX16 | Full-length human PEX16 with an N-terminal EGFP | Matsuzaki and Fujiki, 2008 |
pEGFP-C1/EGFP-PEX16 is based on pEGFP-C1 (Takara Bio Inc.), whereas other plasmids are based on pcDNA3.1/Zeo(+) (Invitrogen).