Table 1.

The primers used in this study

Primer Sequence 
FL-NOD cloning (forward) 5′-GGGGTACCATGGAGGGCGCCAAATTAAGCGCA-3′ 
Motorless NOD cloning (forward) 5′-CGGGGTACCATGCAAGTGGCGCGCCAGAA-3′ 
NOD cloning (reverse) 5′-GGACTAGTAGTGTCGAGACAGTTAATTTGACAAATCGTTC-3′ 
EGFP cloning (forward) 5′-GGACTAGTATGGTGAGCAAGGGCGAGGAG-3′ 
EGFP cloning (reverse no stop codon) 5′-GGCCGCGGCTTGTACAGCTCGTCCATGCC-3′ 
NOD RNAi (forward) 5′-TAATACGACTCACTATAGGGATGGAGGGCGCCAAATTAAG-3′ 
NOD RNAi (reverse) 5′-TAATACGACTCACTATAGGGCGCGCCGCCACCATGGGCAT-3′ 
KLP61F RNAi (forward) 5′-TAATACGACTCACTATAGGGATGTCCAGCGAGGATCCCAG-3′ 
KLP61F RNAi (reverse) 5′-TAATACGACTCACTATAGGGTTGACCTCCCTGATGTCTAC-3′ 
Kinesin1 aa 1–326 cloning (forward) 5′-GCTCTAGAATGGCGGACCTGGCCGAGTG-3′ 
Kinesin1 aa 1–326 cloning (reverse) 5′-ATTGGTACCCTTAATTGTTTTGGCCCTTT-3′ 
EB1 cloning (forward) 5′-GCTCTAGAATGGCTGTAAACGTCTACTC-3′ 
EB1 cloning (reverse) 5′-ATTGGTACCATACTCCTCGTCCTCTGGTG-3′ 
Primer Sequence 
FL-NOD cloning (forward) 5′-GGGGTACCATGGAGGGCGCCAAATTAAGCGCA-3′ 
Motorless NOD cloning (forward) 5′-CGGGGTACCATGCAAGTGGCGCGCCAGAA-3′ 
NOD cloning (reverse) 5′-GGACTAGTAGTGTCGAGACAGTTAATTTGACAAATCGTTC-3′ 
EGFP cloning (forward) 5′-GGACTAGTATGGTGAGCAAGGGCGAGGAG-3′ 
EGFP cloning (reverse no stop codon) 5′-GGCCGCGGCTTGTACAGCTCGTCCATGCC-3′ 
NOD RNAi (forward) 5′-TAATACGACTCACTATAGGGATGGAGGGCGCCAAATTAAG-3′ 
NOD RNAi (reverse) 5′-TAATACGACTCACTATAGGGCGCGCCGCCACCATGGGCAT-3′ 
KLP61F RNAi (forward) 5′-TAATACGACTCACTATAGGGATGTCCAGCGAGGATCCCAG-3′ 
KLP61F RNAi (reverse) 5′-TAATACGACTCACTATAGGGTTGACCTCCCTGATGTCTAC-3′ 
Kinesin1 aa 1–326 cloning (forward) 5′-GCTCTAGAATGGCGGACCTGGCCGAGTG-3′ 
Kinesin1 aa 1–326 cloning (reverse) 5′-ATTGGTACCCTTAATTGTTTTGGCCCTTT-3′ 
EB1 cloning (forward) 5′-GCTCTAGAATGGCTGTAAACGTCTACTC-3′ 
EB1 cloning (reverse) 5′-ATTGGTACCATACTCCTCGTCCTCTGGTG-3′ 

The bases underlined in the RNAi primers represent the T7 promoter sequence. The bases underlined in the cloning primers are restriction sites.

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