Kinetic constants of the interaction of Drg1 and Drg1EQ1 with immobilized Rlp24C (two-state reaction)
| Protein | Nucleotide | kon1 | koff1 | kon2 | koff2 |
| M−1 s−1 | s−1 | s−1 | s−1 | ||
| Drg1 | ATP | 5.4 ± 0.9 × 104 | 1.3 ± 0.1 × 10−1 | 2.8 ± 0.5 × 10−2 | 2.5 ± 0.5 × 10−3 |
| Drg1 | AMP-PNP | 1.1 ± 0.1 × 105 | 6.0 ± 0.9 × 10−2 | 1.5 ± 0.2 × 10−2 | 6.9 ± 0.5 × 10−4 |
| Drg1EQ1 | ATP | 5.8 ± 0.4 × 104 | 5.1 ± 1.5 × 10−3 | 7.2 ± 1.2 × 10−3 | 9.8 ± 0.2 × 10−14 |
| Drg1EQ1 | AMP-PNP | 5.4 ± 0.7 × 104 | 3.5 ± 1.1 × 10−3 | 1.1 ± 0.2 × 10−2 | 8.4 ± 7.4 × 10−14 |
| Protein | Nucleotide | kon1 | koff1 | kon2 | koff2 |
| M−1 s−1 | s−1 | s−1 | s−1 | ||
| Drg1 | ATP | 5.4 ± 0.9 × 104 | 1.3 ± 0.1 × 10−1 | 2.8 ± 0.5 × 10−2 | 2.5 ± 0.5 × 10−3 |
| Drg1 | AMP-PNP | 1.1 ± 0.1 × 105 | 6.0 ± 0.9 × 10−2 | 1.5 ± 0.2 × 10−2 | 6.9 ± 0.5 × 10−4 |
| Drg1EQ1 | ATP | 5.8 ± 0.4 × 104 | 5.1 ± 1.5 × 10−3 | 7.2 ± 1.2 × 10−3 | 9.8 ± 0.2 × 10−14 |
| Drg1EQ1 | AMP-PNP | 5.4 ± 0.7 × 104 | 3.5 ± 1.1 × 10−3 | 1.1 ± 0.2 × 10−2 | 8.4 ± 7.4 × 10−14 |
Mean values determined from five independent injections over at least three different CM5-chips.