Single colonies of antibiotic-resistant transformants were randomly picked and re-streaked on agar plates. A fraction of each colony was resuspended in water or media for light microscopy. The crude flagella preparation was made from a plate of clones randomly selected from the group of Δ1, 1–178, 1–244, and 1–269 that were 100% paralyzed; or from the clones with swimmers from the group of Δ2 and 1–316. The samples were then assessed by HA Western blots. Recorded in each column is the colony number.

The number of colonies that contained swimmers. The percentage of swimmers from each clone varied. The swimmers could not maintain linear trajectory and their flagella had a higher rate of asynchrony than WT cells.

The flagella quantity from each crude preparation for HA Western blots varied, affected by the population of flagellated cells and the deflagellation level.