Table 1. Characterization of Mdv1... | Rockefeller University Press

Table 1.

Characterization of Mdv1 suppressors of the dnm1^F610A allele

Mutations	WT mitochondrial morphology^a		GFP mitochondrial puncta^b		Interaction with Dnm1^c
	DNM1 mdv1Δ	dnm1^F610A mdv1Δ	Dnm1	Dnm1^F610A	Dnm1	Dnm1^F610A
	%	%
Mdv1	67 ± 7	0 ± 0	+	+	+	−
Y418C	6 ± 3	41 ± 16	+	+	++	+
K430R	9 ± 8	28 ± 4	+	+	+	−
E504G	19 ± 8	32 ± 16	+	+	++	−
D539G	9 ± 2	68 ± 11	+	+	++	+
D539Y	11 ± 1	61 ± 14	+	+	++	+
L540P	12 ± 5	58 ± 14	+	+	++	+
S541G	8 ± 4	38 ± 12	+	+	++	+
S557C	9 ± 8	45 ± 8	+	+	++	+
T558I	10 ± 8	34 ± 6	+	+	++	+
D576G	68 ± 17	53 ± 9	+	+	−	−
D585G	45 ± 3	30 ± 8	+	+	−	−
D607G	15 ± 6	31 ± 12	+	+	++	−

Mutations	WT mitochondrial morphologya		GFP mitochondrial punctab		Interaction with Dnm1c
	DNM1 mdv1Δ	dnm1^F610A mdv1Δ	Dnm1	Dnm1^F610A	Dnm1	Dnm1^F610A
	%	%
Mdv1	67 ± 7	0 ± 0	+	+	+	−
Y418C	6 ± 3	41 ± 16	+	+	++	+
K430R	9 ± 8	28 ± 4	+	+	+	−
E504G	19 ± 8	32 ± 16	+	+	++	−
D539G	9 ± 2	68 ± 11	+	+	++	+
D539Y	11 ± 1	61 ± 14	+	+	++	+
L540P	12 ± 5	58 ± 14	+	+	++	+
S541G	8 ± 4	38 ± 12	+	+	++	+
S557C	9 ± 8	45 ± 8	+	+	++	+
T558I	10 ± 8	34 ± 6	+	+	++	+
D576G	68 ± 17	53 ± 9	+	+	−	−
D585G	45 ± 3	30 ± 8	+	+	−	−
D607G	15 ± 6	31 ± 12	+	+	++	−

Numbers are the mean and standard deviation of at least three independent experiments, n = 300.

+, GFP puncta localized to mitochondrial tubules.

Yeast two-hybrid assays. −, No growth on His or Ade minus medium; +, growth on His but not Ade minus medium; ++, growth on both His and Ade minus media. Growth on Ade minus medium is the more stringent indicator of protein–protein interaction.

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