Plasmids used and constructed in this study
| Plasmid | Relevant features | Source |
| pBAD24 | Cloning vector for expression of genes under control of the araBAD promoter | Guzman et al., 1995 |
| pTM123-MBP | pBAD24 producing aa 1–185 of Sco2149 fused to aa 27–396 of E. coli MBP | This study |
| pTM12KK3-MBP | pTM123-MBP with substitution of aa 161–162 of Sco2149 from RR to KK | This study |
| pKK123-MBP | pTM123-MBP with substitution of aa 54 of Sco2149 from R to K | This study |
| p130-MBP | pBAD24, producing aa 130–185 of Sco2149 fused to aa 27–396 of E. coli MBP | This study |
| p148-MBP | pBAD24, producing aa 148–185 of Sco2149 fused to aa 27–396 of E. coli MBP | This study |
| p154-MBP | pBAD24, producing aa 154–185 of Sco2149 fused to aa 27–396 of E. coli MBP | This study |
| p158-MBP | pBAD24, producing aa 158–185 of Sco2149 fused to aa 27–396 of E. coli MBP | This study |
| p158KK-MBP | pBAD24, 158-MBP with exchange of aa 161–162 RR into KK | This study |
| pMltA(2TM)-130-MBP | pBAD24 producing a fusion protein comprising aa 1–86 of E. coli MltA, aa 130–185 of Sco2149 and aa 27–396 of E. coli MBP | This study |
| pMltA(2TM)-TM123-MBP | pBAD24 producing a fusion protein comprising aa 1–86 of E. coli MltA, aa 1–185 of Sco2149 and aa 27–396 of E. coli MBP | This study |
| pSU18 | CmR | Bartolomé et al., 1991 |
| pSUAmiA | pSU18, amiA+ | Ize et al., 2003 |
| pTM123-AmiA | pBAD24 producing aa 1–185 of Sco2149 fused to aa 33–289 of E. coli AmiA | This study |
| pSU-TM123-AmiA | pSU18 producing aa 1–185 of Sco2149 fused to aa 33–289 of E. coli AmiA | This study |
| pTM12KK3-AmiA | pTM123-AmiA with substitution of aa 161–162 of Sco2149 from RR to KK | This study |
| pSU-TM12KK3-AmiA | pSU-TM123-AmiA with substitution of aa 161–162 of Sco2149 from RR to KK | This study |
| psU-TM123-(AxA)AmiA | pSU18 producing aa 1–185 of Sco2149 fused to aa 32–289 of E. coli AmiA | This study |
| pET22b+ | AmpRT7 promoter preceding multiple cloning site | EMD Millipore |
| pET-TM123-AmiA | pET22b, producing aa 1–185 of Sco2149 fused to aa 33–289 of E. coli AmiA | This study |
| pET-TM12KK3-AmiA | pET-TM123-AmiA with substitution of aa 161–162 of Sco2149 from RR to KK | This study |
| pET302 | pTRC99A-derived vector containing lacZR behind the trc promoter, a His tag and an enterokinase site | van der Does et al., 1998 |
| pET2302 | pET302 containing secYEG behind trc promoter with N-terminally His-tagged SecY | de Keyzer et al., 2002 |
| pTRC99A | AmpR lac promoter preceding multiple cloning site | Amann et al., 1988 |
| pTRC99AYidC | pTRC99A carrying yidC | Saller et al., 2009 |
| pQE60 | Cloning vector for producing histidine tagged proteins | QIAGEN |
| pQESco2149 | pQE60 producing Sco2149 with a C-terminal hexa-histidine tag | This study |
| Plasmid | Relevant features | Source |
| pBAD24 | Cloning vector for expression of genes under control of the araBAD promoter | Guzman et al., 1995 |
| pTM123-MBP | pBAD24 producing aa 1–185 of Sco2149 fused to aa 27–396 of E. coli MBP | This study |
| pTM12KK3-MBP | pTM123-MBP with substitution of aa 161–162 of Sco2149 from RR to KK | This study |
| pKK123-MBP | pTM123-MBP with substitution of aa 54 of Sco2149 from R to K | This study |
| p130-MBP | pBAD24, producing aa 130–185 of Sco2149 fused to aa 27–396 of E. coli MBP | This study |
| p148-MBP | pBAD24, producing aa 148–185 of Sco2149 fused to aa 27–396 of E. coli MBP | This study |
| p154-MBP | pBAD24, producing aa 154–185 of Sco2149 fused to aa 27–396 of E. coli MBP | This study |
| p158-MBP | pBAD24, producing aa 158–185 of Sco2149 fused to aa 27–396 of E. coli MBP | This study |
| p158KK-MBP | pBAD24, 158-MBP with exchange of aa 161–162 RR into KK | This study |
| pMltA(2TM)-130-MBP | pBAD24 producing a fusion protein comprising aa 1–86 of E. coli MltA, aa 130–185 of Sco2149 and aa 27–396 of E. coli MBP | This study |
| pMltA(2TM)-TM123-MBP | pBAD24 producing a fusion protein comprising aa 1–86 of E. coli MltA, aa 1–185 of Sco2149 and aa 27–396 of E. coli MBP | This study |
| pSU18 | CmR | Bartolomé et al., 1991 |
| pSUAmiA | pSU18, amiA+ | Ize et al., 2003 |
| pTM123-AmiA | pBAD24 producing aa 1–185 of Sco2149 fused to aa 33–289 of E. coli AmiA | This study |
| pSU-TM123-AmiA | pSU18 producing aa 1–185 of Sco2149 fused to aa 33–289 of E. coli AmiA | This study |
| pTM12KK3-AmiA | pTM123-AmiA with substitution of aa 161–162 of Sco2149 from RR to KK | This study |
| pSU-TM12KK3-AmiA | pSU-TM123-AmiA with substitution of aa 161–162 of Sco2149 from RR to KK | This study |
| psU-TM123-(AxA)AmiA | pSU18 producing aa 1–185 of Sco2149 fused to aa 32–289 of E. coli AmiA | This study |
| pET22b+ | AmpRT7 promoter preceding multiple cloning site | EMD Millipore |
| pET-TM123-AmiA | pET22b, producing aa 1–185 of Sco2149 fused to aa 33–289 of E. coli AmiA | This study |
| pET-TM12KK3-AmiA | pET-TM123-AmiA with substitution of aa 161–162 of Sco2149 from RR to KK | This study |
| pET302 | pTRC99A-derived vector containing lacZR behind the trc promoter, a His tag and an enterokinase site | van der Does et al., 1998 |
| pET2302 | pET302 containing secYEG behind trc promoter with N-terminally His-tagged SecY | de Keyzer et al., 2002 |
| pTRC99A | AmpR lac promoter preceding multiple cloning site | Amann et al., 1988 |
| pTRC99AYidC | pTRC99A carrying yidC | Saller et al., 2009 |
| pQE60 | Cloning vector for producing histidine tagged proteins | QIAGEN |
| pQESco2149 | pQE60 producing Sco2149 with a C-terminal hexa-histidine tag | This study |