Strains and plasmids used in this paper
| Strain name | Genotype | Reference |
| FB2 | a2b2 | (Banuett and Herskowitz, 1989) |
| FB2N107G | a2b2 Pnup107-nup107-egfp, bleR | This paper |
| FB2N107G_nR | a2b2 Pnup107-nup107-gfp, bleR/pH_NLS3xRFP | (Theisen et al., 2008) |
| Nup82-GFP | MATa his3 ura3Δ0 leuΔ0 met15Δ0 NUP82-GFP::kanR | Invitrogen |
| CDS234 | Nup133-GFP::pyrGAf; pyrG89; pyroA4; wA3 | (De Souza et al., 2004) |
| FB2nRFP_GT | a2b2 / pH_NLS3xRFP/pGFPTub1 | (Straube et al., 2005) |
| FB2N107G_RT | a2b2 Pnup107- nup107-gfp, bleR/pN_RFPTub1 | (Theisen et al., 2008) |
| FB2N133G | a2b2 Pnup133-nup133-gfp, natR | This paper |
| FB2N133G_nR | a2b2 Pnup133-nup133-GFP, natR/pC_NLS3xRFP | This paper |
| FB2ΔKin1_N133G_nR | a2b2 Δkin1::hygR Pnup133-nup133-GFP, natR/pC_NLS3xRFP | This paper |
| FB2Dyn2ts_N107G_nR | a2b2 dyn2ts natR, Pnup107-nup107-gfp, bleR/pC_NLS3xRFP | This paper |
| FB2ΔKin1_rDyn2_N133G | a2b2 Δkin1::hygR Pcrg-dyn2, bleR Pnup133-nup133-GFP, natR | This paper |
| FB2G3Dyn2_N107R | a2b2 Pnup107-nup107-rfp, bleR Pdyn2-3xgfp-dyn2, hygR | This paper |
| FB2nR_H4G | a2b2 / pC_NLS3xRFP / pH4GFP | This paper |
| FB2N107G_rH4mCh | a2b2 Pnup107-nup107-egfp, bleR/PrH4-cH | This paper |
| FB2N107R_nlsGFP | a2b2 Pnup107nup107-rfp, bleR/pnGFP | This paper |
| FB2ΔKin1_N107R_nG | a2b2 Δkin1::hygR Pnup107-nup107rfp, bleR/pnGFP | This paper |
| FB1rDyn2_N214R_nG | a1b1 Pcrg-dyn2, bleR Pnup214-nup214-rfp, hygR/pnGFP | This paper |
| FB2ΔKin1_rDyn2_N107R_nG | a2b2 Δkin1::hygR Pnup107nup-nup107rfp, bleR Pcrg-dyn2, natR/pnGFP | This paper |
| FB2N107R_NLS-NES-paG | a2b2 Pnup107-nup107-rfp, bleR/pC_NLS-NES-paGFP | This paper |
| FB2ΔKin1_N107R_NLS-NES-paG | a2b2 Δkin1::hygR Pnup107-nup107rfp, bleR/pC_NLS-NES-paGFP | This paper |
| FB1rDyn2_P152R_NLS-NES-paG | a1b1 Pcrg-dyn2, bleR Ppom152-pom152-rfp, hygR/pC_NLS-NES-paGFP | This paper |
| FB1ΔKin1_rDyn2_P152R_NLS-NES-pG | a1b1 Δkin1::natR Pcrg-dyn2, bleR Ppom152-pom152-rfp, hygR/pC_NLS-NES-paGFP | This paper |
| FB2rN107_N214G | a2b2 Pnup214-nup214-gfp, hygR Pcrg-nup107, ble R | This paper |
| AB31nNudE_N133G | a2 PcrgbW2 PcrgbE1, Pnar-nde1, hygR, Pnup133-nup133-gfp, natR | This paper |
| pH_NLS3xRFP | Potef-gal4s-mrfp-mrfp-mrfp, hygR | (Straube et al., 2005) |
| PrH4-cH | Pcrg-his4-mcherry, cbxR | This paper |
| pGFPTub1 | Potef-gfp-tub1, cbxR | (Steinberg et al., 2001) |
| pN_RFPTub1 | Potef-2xmrfp-tub1, natR | (Theisen et al., 2008) |
| pERRFP | Potef-cals-mrfp-HDEL, cbxR | (Theisen et al., 2008) |
| pC_NLS3xRFP | Potef-gal4s-mrfp-mrfp-mrfp, cbxR | (Straube et al., 2005) |
| pnGFP | gal4(s)::egfp, cbx | (Straube et al., 2001) |
| pC_NLS-NES-paGFP | Potef-MARVS-NES-pagfp, cbxR | This paper |
| pH4GFP | Potef-his4-gfp, hygR | (Straube et al., 2001) |
| Strain name | Genotype | Reference |
| FB2 | a2b2 | (Banuett and Herskowitz, 1989) |
| FB2N107G | a2b2 Pnup107-nup107-egfp, bleR | This paper |
| FB2N107G_nR | a2b2 Pnup107-nup107-gfp, bleR/pH_NLS3xRFP | (Theisen et al., 2008) |
| Nup82-GFP | MATa his3 ura3Δ0 leuΔ0 met15Δ0 NUP82-GFP::kanR | Invitrogen |
| CDS234 | Nup133-GFP::pyrGAf; pyrG89; pyroA4; wA3 | (De Souza et al., 2004) |
| FB2nRFP_GT | a2b2 / pH_NLS3xRFP/pGFPTub1 | (Straube et al., 2005) |
| FB2N107G_RT | a2b2 Pnup107- nup107-gfp, bleR/pN_RFPTub1 | (Theisen et al., 2008) |
| FB2N133G | a2b2 Pnup133-nup133-gfp, natR | This paper |
| FB2N133G_nR | a2b2 Pnup133-nup133-GFP, natR/pC_NLS3xRFP | This paper |
| FB2ΔKin1_N133G_nR | a2b2 Δkin1::hygR Pnup133-nup133-GFP, natR/pC_NLS3xRFP | This paper |
| FB2Dyn2ts_N107G_nR | a2b2 dyn2ts natR, Pnup107-nup107-gfp, bleR/pC_NLS3xRFP | This paper |
| FB2ΔKin1_rDyn2_N133G | a2b2 Δkin1::hygR Pcrg-dyn2, bleR Pnup133-nup133-GFP, natR | This paper |
| FB2G3Dyn2_N107R | a2b2 Pnup107-nup107-rfp, bleR Pdyn2-3xgfp-dyn2, hygR | This paper |
| FB2nR_H4G | a2b2 / pC_NLS3xRFP / pH4GFP | This paper |
| FB2N107G_rH4mCh | a2b2 Pnup107-nup107-egfp, bleR/PrH4-cH | This paper |
| FB2N107R_nlsGFP | a2b2 Pnup107nup107-rfp, bleR/pnGFP | This paper |
| FB2ΔKin1_N107R_nG | a2b2 Δkin1::hygR Pnup107-nup107rfp, bleR/pnGFP | This paper |
| FB1rDyn2_N214R_nG | a1b1 Pcrg-dyn2, bleR Pnup214-nup214-rfp, hygR/pnGFP | This paper |
| FB2ΔKin1_rDyn2_N107R_nG | a2b2 Δkin1::hygR Pnup107nup-nup107rfp, bleR Pcrg-dyn2, natR/pnGFP | This paper |
| FB2N107R_NLS-NES-paG | a2b2 Pnup107-nup107-rfp, bleR/pC_NLS-NES-paGFP | This paper |
| FB2ΔKin1_N107R_NLS-NES-paG | a2b2 Δkin1::hygR Pnup107-nup107rfp, bleR/pC_NLS-NES-paGFP | This paper |
| FB1rDyn2_P152R_NLS-NES-paG | a1b1 Pcrg-dyn2, bleR Ppom152-pom152-rfp, hygR/pC_NLS-NES-paGFP | This paper |
| FB1ΔKin1_rDyn2_P152R_NLS-NES-pG | a1b1 Δkin1::natR Pcrg-dyn2, bleR Ppom152-pom152-rfp, hygR/pC_NLS-NES-paGFP | This paper |
| FB2rN107_N214G | a2b2 Pnup214-nup214-gfp, hygR Pcrg-nup107, ble R | This paper |
| AB31nNudE_N133G | a2 PcrgbW2 PcrgbE1, Pnar-nde1, hygR, Pnup133-nup133-gfp, natR | This paper |
| pH_NLS3xRFP | Potef-gal4s-mrfp-mrfp-mrfp, hygR | (Straube et al., 2005) |
| PrH4-cH | Pcrg-his4-mcherry, cbxR | This paper |
| pGFPTub1 | Potef-gfp-tub1, cbxR | (Steinberg et al., 2001) |
| pN_RFPTub1 | Potef-2xmrfp-tub1, natR | (Theisen et al., 2008) |
| pERRFP | Potef-cals-mrfp-HDEL, cbxR | (Theisen et al., 2008) |
| pC_NLS3xRFP | Potef-gal4s-mrfp-mrfp-mrfp, cbxR | (Straube et al., 2005) |
| pnGFP | gal4(s)::egfp, cbx | (Straube et al., 2001) |
| pC_NLS-NES-paGFP | Potef-MARVS-NES-pagfp, cbxR | This paper |
| pH4GFP | Potef-his4-gfp, hygR | (Straube et al., 2001) |
a, b: mating type loci; P: promoter; -: fusion; hygR: hygromycin resistance; bleR: phleomycin resistance; natR: nourseothricin resistance; cbxR: carboxin resistance; ts: temperature-sensitive allele; /: ectopically integrated; crg: conditional arabinose-induced promoter; otef: constitutive promoter; nup107, nup133, nup214, nup84, NUP82: nucleoporins; pom152: integral pore membrane protein; HDEL: ER retention signal; egfp: enhanced GFP; rfp: monomeric RFP; mcherry: monomeric cherry, paGFP: photoactivatable GFP; dyn2: C-terminal half of the dynein heavy chain; dyn1: N-terminal half of the dynein heavy chain; tub1: α-tubulin; kin1: kinesin1; gal4s: nuclear localization signal; MARVS: nuclear localization signal; NES, nuclear export sequence of protein kinase Fuz7 (um01514); pyrGAf: pyrG gene of Aspergillus fumigatus; pyrG89: A. nidulans uridine/uracil auxotrophic marker; pyroA4: A. nidulans pyridoxine auxotrophic marker; wA3: A. nidulans mutant allele causing white conidia; argB2: A. nidulans arginine auxotrophic marker; nirA14: A. nidulans mutant allele preventing nitrate utilization; sE15: A. nidulans thiosulfate auxotrophic marker; MATa: S. cerevisiae mating locus; his3, ura3Δ0, leuΔ0, met15Δ: S. cerevisiae auxotrophic markers; kanR: kanmycin resistance.