Table 2. Quantification of the... | Rockefeller University Press

Table 2.

Quantification of the colocalization of AGO2 with Gag in HIV-1 infected cells

	Experiment 1		Experiment 2		Experiment 1 and 2 combined		Fold colocalization
	WT Gag	GagΔp2-NC-p1-p6	WT Gag	GagΔp2-NC-p1-p6	WT Gag	GagΔp2-NC-p1-p6 total	WT Gag	GagΔp2-NC-p1-p6 total
Membrane length (µm)	178	174	158	168	336	342
Total AGO2 label	387	442	1,006	544	1,393	986
Total targeted Gag clusters	106	461	80	358	186	819
Targeted Gag clusters with AGO2	19	95	27	90	46	185	1.11	(1.00)
Gag clusters at early assembly sites	27	0	55	0	82	0
Gag clusters at early assembly sites with AGO2	12	n/a	38	n/a	50	n/a	2.45^a
Gag clusters at late assembly sites	102	0	43	0	145	0
Gag clusters at late assembly sites with AGO2	41	n/a	31	n/a	72	n/a	2.41^b

	Experiment 1		Experiment 2		Experiment 1 and 2 combined		Fold colocalization
	WT Gag	GagΔp2-NC-p1-p6	WT Gag	GagΔp2-NC-p1-p6	WT Gag	GagΔp2-NC-p1-p6 total	WT Gag	GagΔp2-NC-p1-p6 total
Membrane length (µm)	178	174	158	168	336	342
Total AGO2 label	387	442	1,006	544	1,393	986
Total targeted Gag clusters	106	461	80	358	186	819
Targeted Gag clusters with AGO2	19	95	27	90	46	185	1.11	(1.00)
Gag clusters at early assembly sites	27	0	55	0	82	0
Gag clusters at early assembly sites with AGO2	12	n/a	38	n/a	50	n/a	2.45^a
Gag clusters at late assembly sites	102	0	43	0	145	0
Gag clusters at late assembly sites with AGO2	41	n/a	31	n/a	72	n/a	2.41^b

Cells infected with the indicated proviruses were double immunogold labeled for Gag and AGO2 and analyzed as described for Table 1. Fold colocalization is defined as the number of WT Gag clusters colocalized with AGO2 for each stage of assembly, normalized to Gag-AGO2 colocalization in the GagΔp2-NC-p1-p6 negative control (indicated by parentheses). Fold colocalization (bold) with SD is graphed in Fig. 6.

P < 0.05.

P < 0.1.

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