Kinetic parameters of microtubule polymerization dynamics in the EBN-Sentin-GFP rescue experiment
| Parameters | Control | EB1–Sentin RNAi | |
| GFP not expressed | GFP expressed | ||
| Growth rate (µm/min) | 4.5 ± 0.7 | 4.1 ± 0.4 | 4.3 ± 0.9 |
| 4.6 ± 1.7m | 4.0 ± 1.7m | 4.3 ± 1.6m | |
| Shrink rate (µm/min) | 10.6 ± 2.3 | 9.6 ± 3.4 | 8.4 ± 0.9 |
| 10.9 ± 5.3m | 9.9 ± 5.9m | 8.4 ± 3.3m | |
| Catastrophe frequency (s−1) | 0.012 ± 0.004 | 0.006 ± 0.004 | 0.016 ± 0.004 |
| Rescue frequency (s−1) | 0.005 ± 0.002 | 0.002 ± 0.002 | 0.010 ± 0.005 |
| Time in growth (%) | 39.3 ± 12.1 | 11.5 ± 7.6 | 36.4 ± 7.9 |
| Time in shrink (%) | 14.1 ± 4.4 | 5.4 ± 4.3 | 16.8 ± 5.3 |
| Time in pause (%) | 46.6 ± 13.2 | 83.1 ± 11.1 | 46.8 ± 11.8 |
| Parameters | Control | EB1–Sentin RNAi | |
| GFP not expressed | GFP expressed | ||
| Growth rate (µm/min) | 4.5 ± 0.7 | 4.1 ± 0.4 | 4.3 ± 0.9 |
| 4.6 ± 1.7m | 4.0 ± 1.7m | 4.3 ± 1.6m | |
| Shrink rate (µm/min) | 10.6 ± 2.3 | 9.6 ± 3.4 | 8.4 ± 0.9 |
| 10.9 ± 5.3m | 9.9 ± 5.9m | 8.4 ± 3.3m | |
| Catastrophe frequency (s−1) | 0.012 ± 0.004 | 0.006 ± 0.004 | 0.016 ± 0.004 |
| Rescue frequency (s−1) | 0.005 ± 0.002 | 0.002 ± 0.002 | 0.010 ± 0.005 |
| Time in growth (%) | 39.3 ± 12.1 | 11.5 ± 7.6 | 36.4 ± 7.9 |
| Time in shrink (%) | 14.1 ± 4.4 | 5.4 ± 4.3 | 16.8 ± 5.3 |
| Time in pause (%) | 46.6 ± 13.2 | 83.1 ± 11.1 | 46.8 ± 11.8 |
Means ± SD. SD represents either microtubule to-microtubule (marked with an m) or cell to cell (others) variability (80 microtubules and 5 cells).