Table II.

Kinetic parameters of centrosomal microtubule polymerization dynamics in mitosis

Parameters RNAi 
 Control Sentin Kinesin-13Klp10A 
Growth rate (µm/min) 10.8 ± 1.6 8.8 ± 2.4 9.8 ± 1.3 
 10.8 ± 2.1m 8.7 ± 4.0m 9.7 ± 2.5m 
Shrink rate (µm/min) 19.6 ± 4.4 21.6 ± 4.9 40.2 ± 8.7 
 19.5 ± 5.2m 21.7 ± 6.5m 40.2 ± 12.0m 
Catastrophe frequency (s−10.035 ± 0.006 0.021 ± 0.008 0.025 ± 0.007 
Time in growth (%) 53.1 ± 8.6 34.7 ± 15.3 62.7 ± 8.4 
Time in shrink (%) 32.0 ± 7.5 17.8 ± 7.3 18.9 ± 6.0 
Time in pause (%) 14.9 ± 11.1 47.5 ± 17.7 18.4 ± 10.6 
Parameters RNAi 
 Control Sentin Kinesin-13Klp10A 
Growth rate (µm/min) 10.8 ± 1.6 8.8 ± 2.4 9.8 ± 1.3 
 10.8 ± 2.1m 8.7 ± 4.0m 9.7 ± 2.5m 
Shrink rate (µm/min) 19.6 ± 4.4 21.6 ± 4.9 40.2 ± 8.7 
 19.5 ± 5.2m 21.7 ± 6.5m 40.2 ± 12.0m 
Catastrophe frequency (s−10.035 ± 0.006 0.021 ± 0.008 0.025 ± 0.007 
Time in growth (%) 53.1 ± 8.6 34.7 ± 15.3 62.7 ± 8.4 
Time in shrink (%) 32.0 ± 7.5 17.8 ± 7.3 18.9 ± 6.0 
Time in pause (%) 14.9 ± 11.1 47.5 ± 17.7 18.4 ± 10.6 

Means ± SD. SD represents either microtubule to microtubule (marked with an m) or cell to cell (others) variability (20 microtubules and 10 cells).

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