Table I.

Kinetic parameters of microtubule polymerization dynamics after Sentin RNAi in S2 cells

Parameters RNAi 
 Control EB1 Sentin 
Growth rate (µm/min) 5.3 ± 1.4 4.5 ± 0.8 3.5 ± 0.5 
 5.3 ± 3.1m 4.4 ± 2.5m 3.5 ± 2.0m 
Shrink rate (µm/min) 11.3 ± 1.9 7.9 ± 1.4 7.7 ± 2.0 
 11.1 ± 6.6m 7.5 ± 4.9m 7.4 ± 6.0m 
Catastrophe frequency (s−10.011 ± 0.003 0.005 ± 0.001 0.005 ± 0.001 
Rescue frequency (s−10.005 ± 0.002 0.002 ± 0.001 0.003 ± 0.001 
Time in growth (%) 39.0 ± 11.5 14.1 ± 8.2 20.8 ± 11.1 
Time in shrink (%) 19.7 ± 5.0 7.9 ± 3.1 8.0 ± 1.6 
Time in pause (%) 41.3 ± 10.5 78.0 ± 10.8 71.2 ± 11.7 
Parameters RNAi 
 Control EB1 Sentin 
Growth rate (µm/min) 5.3 ± 1.4 4.5 ± 0.8 3.5 ± 0.5 
 5.3 ± 3.1m 4.4 ± 2.5m 3.5 ± 2.0m 
Shrink rate (µm/min) 11.3 ± 1.9 7.9 ± 1.4 7.7 ± 2.0 
 11.1 ± 6.6m 7.5 ± 4.9m 7.4 ± 6.0m 
Catastrophe frequency (s−10.011 ± 0.003 0.005 ± 0.001 0.005 ± 0.001 
Rescue frequency (s−10.005 ± 0.002 0.002 ± 0.001 0.003 ± 0.001 
Time in growth (%) 39.0 ± 11.5 14.1 ± 8.2 20.8 ± 11.1 
Time in shrink (%) 19.7 ± 5.0 7.9 ± 3.1 8.0 ± 1.6 
Time in pause (%) 41.3 ± 10.5 78.0 ± 10.8 71.2 ± 11.7 

Means ± SD. SD represents either microtubule to microtubule (marked with an m) or cell to cell (others) variability. n = 84 microtubules and 5 cells for control, 83 microtubules and 5 cells for EB1, and 80 microtubules and 5 cells for Sentin.

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