Regulation of FGFR1 target genes
| Gene | FGFR1b-VSAD | FGFR1 RNAi | FGFR1b-VSAN | |||
| Fold change | P-value | Fold change | P-value | Fold change | P-value | |
| EBI3 | 0.25 ± 0.02 | <0.001 | 1.93 ± 0.26 | <0.05 | 1.07 ± 0.39 | NS |
| GRINA | 0.7 ± 0.14 | <0.05 | 7.38 ± 5.5 | NS | 1.55 ± 0.57 | NS |
| KRTAP5-6 | 4.25 ± 1.66 | NS | 0.27 ± 0.05 | <0.01 | 0.77 ± 0.27 | NS |
| POU2F2 | 2.42 ± 1.3 | NS | 1.34 ± 0.35 | NS | − | − |
| PRSS27 | 3.25 ± 1.6 | NS | 0.31 ± 0.05 | <0.01 | 1.53 ± 0.63 | NS |
| SFN | 3.76 ± 1.56 | <0.05 | 0.57 ± 0.11 | <0.05 | 1.17 ± 0.26 | NS |
| STAC3 | 0.75 ± 0.13 | <0.05 | 0.5 ± 0.12 | <0.01 | − | − |
| Gene | FGFR1b-VSAD | FGFR1 RNAi | FGFR1b-VSAN | |||
| Fold change | P-value | Fold change | P-value | Fold change | P-value | |
| EBI3 | 0.25 ± 0.02 | <0.001 | 1.93 ± 0.26 | <0.05 | 1.07 ± 0.39 | NS |
| GRINA | 0.7 ± 0.14 | <0.05 | 7.38 ± 5.5 | NS | 1.55 ± 0.57 | NS |
| KRTAP5-6 | 4.25 ± 1.66 | NS | 0.27 ± 0.05 | <0.01 | 0.77 ± 0.27 | NS |
| POU2F2 | 2.42 ± 1.3 | NS | 1.34 ± 0.35 | NS | − | − |
| PRSS27 | 3.25 ± 1.6 | NS | 0.31 ± 0.05 | <0.01 | 1.53 ± 0.63 | NS |
| SFN | 3.76 ± 1.56 | <0.05 | 0.57 ± 0.11 | <0.05 | 1.17 ± 0.26 | NS |
| STAC3 | 0.75 ± 0.13 | <0.05 | 0.5 ± 0.12 | <0.01 | − | − |
Fold change of mRNA expression in MCF-7 cells of genes identified after ChIP on chip with an anti-FGFR1 antibody, after 48-h overexpression of WT (VSAD) FGFR1b, 48-h RNAi of FGFR1, or 48-h overexpression of GrB-resistant (VSAN) FGFR1b. Real-time PCR was analyzed using the 2−ΔΔCt method and using expression of HPRT and GAPDH as housekeeping genes for normalization. Data presented in the table are normalized with GAPDH. n ≥ 3 independent experiments in triplicate. Minus signs indicate where analysis was not performed. Statistical significance was tested with both Mann–Whitney and Student’s t test.