Effect of RLC-D,D–GFP, 50 μM blebbistatin, and 5 μM nocodazole on proto-bundle formation in cells spreading on fibronectin or attached to PLL
| Condition | Fibronectin (10 min) | PLL |
| Control (wild-type RLC) | 48 ± 1 | 52 ± 10 |
| RLC-D,D | 60 ± 7a | 73 ± 12a |
| Blebbistatin | 1.5 ± 0.5 | 1 ± 2 |
| Nocodazole | 41 ± 8 | 59 ± 5 |
| Condition | Fibronectin (10 min) | PLL |
| Control (wild-type RLC) | 48 ± 1 | 52 ± 10 |
| RLC-D,D | 60 ± 7a | 73 ± 12a |
| Blebbistatin | 1.5 ± 0.5 | 1 ± 2 |
| Nocodazole | 41 ± 8 | 59 ± 5 |
Data represent the percentage of cells displaying an F-actin–enriched proto-bundle, and are expressed as mean ± SD of three experiments. >200 cells were scored in each experiment.
p < 0.05, Student’s t test (RLC-D,D vs. control).