Quantification of various ciliary ultrastructure defects in TZ protein–disrupted strains
| Region | Feature/defect | N2 | mks-6 | mks-6; nphp-4 | mksr-1; nphp-4 | mks-1; nphp-4 | mks-5; nphp-4 | mks-3; nphp-4 |
| DSa | Ave. axoneme no./pore (n = 2–4) | 10 | 10 | 3 | 2 | 6 | 5 | 5 |
| Ave. MT singlet no./axoneme (n = 3–44) | 8 | 7 | 5 | 7 | 8 | 4 | 6 | |
| No. of axonemes with no MTsd | 0 | 4 | 9 | 3 | 8 | 3 | 2 | |
| MSb | Ave. axoneme no./pore (n = 2–4) | 12 | 12 | 5 | 4 | 11 | 9 | 7 |
| Ave. MT doublet no./axoneme (n = 12–100) | 9 | 8.9 | 7.7 | 6.0 | 8.3 | 8.0 | 7.5 | |
| Axonemes with abnormal accumulationsde | 0 | 0 | 10 | 0 | 0 | 2 | 0 | |
| TZc | Ave. diameter of surrounding membrane (nm) (n′ = 2–34) | 260 | 262 | 564 | 436 | 286 | 337 | 320 |
| Percentage with enlarged membrane diameters (n′ = 2–34) | 0 | 0 | 100 | 100 | 25 | 100 | 60 | |
| Percentage of TZs with Y-links (n′ = 2–14) | 100 | 100 | 0 | 0 | 50 | 0 | 30 | |
| DD | Abnormal vesicle or membrane accumulationsde | 0 | 0 | 23 | 4 | 2 | 5 | 4 |
| Region | Feature/defect | N2 | mks-6 | mks-6; nphp-4 | mksr-1; nphp-4 | mks-1; nphp-4 | mks-5; nphp-4 | mks-3; nphp-4 |
| DSa | Ave. axoneme no./pore (n = 2–4) | 10 | 10 | 3 | 2 | 6 | 5 | 5 |
| Ave. MT singlet no./axoneme (n = 3–44) | 8 | 7 | 5 | 7 | 8 | 4 | 6 | |
| No. of axonemes with no MTsd | 0 | 4 | 9 | 3 | 8 | 3 | 2 | |
| MSb | Ave. axoneme no./pore (n = 2–4) | 12 | 12 | 5 | 4 | 11 | 9 | 7 |
| Ave. MT doublet no./axoneme (n = 12–100) | 9 | 8.9 | 7.7 | 6.0 | 8.3 | 8.0 | 7.5 | |
| Axonemes with abnormal accumulationsde | 0 | 0 | 10 | 0 | 0 | 2 | 0 | |
| TZc | Ave. diameter of surrounding membrane (nm) (n′ = 2–34) | 260 | 262 | 564 | 436 | 286 | 337 | 320 |
| Percentage with enlarged membrane diameters (n′ = 2–34) | 0 | 0 | 100 | 100 | 25 | 100 | 60 | |
| Percentage of TZs with Y-links (n′ = 2–14) | 100 | 100 | 0 | 0 | 50 | 0 | 30 | |
| DD | Abnormal vesicle or membrane accumulationsde | 0 | 0 | 23 | 4 | 2 | 5 | 4 |
DS, distal segment; MS, middle segment; TZ, transition zone; DD, distal dendrite; N, number of amphid pores analyzed; n, number of axonemes measured; n′, number of TZs analyzed.
Singlet microtubule (MT) axoneme region.
Doublet MT axoneme region.
Drawing together of doublet and singlet MTs by the apical membrane; doublets linked to ciliary membrane via Y-links.
As analyses were performed on serial sections, the same axoneme may have been counted multiple times.
Electron-dense accumulations, often vesicular (30–60 nm; often tubular; clear or dense core), sometimes non vesicular (<20 nm).