Replication events in telomeric chromosomal segments analyzed by SMARD
| Telomere segment of chromosome | Approximate size of the segment | Cell type | Replication events | |||
| Initiation | 5′-to-3′ fork | 3′-to-5′ fork | Termination | |||
| 5p | 200 kb | ES (H1) | 10 (24%) | 18 (43%) | 14 (33%) | 0 (0%) |
| 5p | 200 kb | ES (H9) | 10 (26%) | 11 (28%) | 17 (44%) | 1 (2%) |
| 5p | 200 kb | Lung fibroblast (IMR-90) | 3 (15%) | 15 (75%) | 2 (10%) | 0 (0%) |
| 7q | 200 kb | ES (H9) | 3 (8%) | 18 (49%) | 12 (32%) | 4 (11%) |
| 10q | 210 kb | ES (H1) | 0 (0%) | 28 (97%) | 0 (0%) | 1 (3%) |
| 10q | 210 kb | ES (H9) | 2 (8%) | 17 (68%) | 2 (8%) | 4 (16%) |
| 10q | 270 kb | Lung fibroblast (IMR-90) | 0 (0%) | 19 (100%) | 0 (0%) | 0 (0%) |
| 10q | 210 kb | MEC | 2 (6%) | 22 (65%) | 2 (6%) | 8 (23%) |
| 10q | 240 kb | HeLa 1.3 | 3 (13%) | 13 (54%) | 2 (8%) | 6 (25%) |
| 11q | 140 kb | ES (H1) | 19 (32%) | 22 (37%) | 16 (27%) | 2 (4%) |
| 11q | 140 kb | ES (H9) | 17 (36%) | 21 (45%) | 7 (15%) | 2 (4%) |
| 11q | 140 kb | MEC | 6 (25%) | 12 (50%) | 3 (12.5%) | 3 (12.5%) |
| Telomere segment of chromosome | Approximate size of the segment | Cell type | Replication events | |||
| Initiation | 5′-to-3′ fork | 3′-to-5′ fork | Termination | |||
| 5p | 200 kb | ES (H1) | 10 (24%) | 18 (43%) | 14 (33%) | 0 (0%) |
| 5p | 200 kb | ES (H9) | 10 (26%) | 11 (28%) | 17 (44%) | 1 (2%) |
| 5p | 200 kb | Lung fibroblast (IMR-90) | 3 (15%) | 15 (75%) | 2 (10%) | 0 (0%) |
| 7q | 200 kb | ES (H9) | 3 (8%) | 18 (49%) | 12 (32%) | 4 (11%) |
| 10q | 210 kb | ES (H1) | 0 (0%) | 28 (97%) | 0 (0%) | 1 (3%) |
| 10q | 210 kb | ES (H9) | 2 (8%) | 17 (68%) | 2 (8%) | 4 (16%) |
| 10q | 270 kb | Lung fibroblast (IMR-90) | 0 (0%) | 19 (100%) | 0 (0%) | 0 (0%) |
| 10q | 210 kb | MEC | 2 (6%) | 22 (65%) | 2 (6%) | 8 (23%) |
| 10q | 240 kb | HeLa 1.3 | 3 (13%) | 13 (54%) | 2 (8%) | 6 (25%) |
| 11q | 140 kb | ES (H1) | 19 (32%) | 22 (37%) | 16 (27%) | 2 (4%) |
| 11q | 140 kb | ES (H9) | 17 (36%) | 21 (45%) | 7 (15%) | 2 (4%) |
| 11q | 140 kb | MEC | 6 (25%) | 12 (50%) | 3 (12.5%) | 3 (12.5%) |
A summary of all replication events detected in the SMARD analysis of telomere/subtelomere segments (Figs. 2–6) from the various cell types studied is shown. The number of fully IdU- and CldU-substituted (red-green) molecules containing initiations, terminations, or a single visible fork (5′ to 3′ or 3′ to 5′) is indicated. The percentage of a given event of the total events observed is indicated in parentheses.