Node protein dynamics revealed by FRAP and numbers of molecules in nodes
| Genotype | Half-time, fast/slow phase (number of cells) | Recovery, fast/slow | Molecules (polypeptides) per node, first/second plateau (number of nodes) | Timing of node appearance (number of nodes) |
| min | % | min | ||
| mid1-mYFP | ND | ND | 17.1 ± 7.4 (4)/27.7 ± 10 (23) | −11.2 ± 1.1 (9) |
| mid1-mECitrine | 3.03 ± 1.32 (10) | 68.6 | ND | ND |
| mid1-mECitrine cdr2Δ | 1.97 ± 0.77 (10) | 57.8 | ND | ND |
| mYFP-rng2 | 1.77 ± 1.07 (14) | 75.4 | 9.4 ± 5.6 (25)/28.0 ± 16.9 (25) | −12.5 ± 1.1 (8) |
| mYFP-rng2 myo2-IQ1ΔIQ2Δ | 1.39 ± 0.82 (10) | 56.7 | 10.3 ± 3.4 (16)/30.3 ± 9.4 (16)a | −12.8 ± 1.0 (16)a |
| mYFP-rng2 cdr2Δ | 1.29 ± 0.69 (14) | 57.0 | ND | ND |
| mYFP-cdc4 | 0.20 ± 0.14/1.45 ± 0.45 (11) | 21.1/22.3 | 134.1 ± 93.9 (29)/286.3 ± 130.4 (29) | −11.9 ± 1.4 (11) |
| mYFP-cdc4 myo2-IQ1ΔIQ2Δ | NA/1.39 ± 0.89 (24) | NA/53.4 | 107.5 ± 59.7 (16)/265.0 ± 90.1 (16)a | −11.4 ± 1.5 (16)a |
| mYFP-myo2 | 0.51 ± 0.22 (14) | 59.5 | 55.3 ± 32.8 (31) | −10.2 ± 2.5 (9) |
| mYFP-myo2-IQ1ΔIQ2Δ | 0.48 ± 0.31 (18) | 71.4 | ND | ND |
| mYFP-myo2 cdr2Δ | 0.52 ± 0.22 (11) | 79.7 | ND | ND |
| rlc1-mYFP | 0.51 ± 0.22 (18) | 61.9 | 41.3 ± 23 (31) | −10.2 ± 1.8 (14) |
| mYFP-cdc15 | 0.07 ± 0.02/1.10 ± 0.67 (15) | 57.3/27.3 | 36.3 ± 16.9 (21) | −4.9 ± 0.6 (12) |
| mYFP-cdc15 cdr2Δ | 0.08 ± 0.04/0.83 ± 0.62 (15) | 50.9/49.1 | ND | ND |
| cdc12-3YFPb | 0.50 ± 0.17 (11) | 69.3 | 6.9 ± 2.9 (16) | 1.0 ± 0.6 (16) |
| Genotype | Half-time, fast/slow phase (number of cells) | Recovery, fast/slow | Molecules (polypeptides) per node, first/second plateau (number of nodes) | Timing of node appearance (number of nodes) |
| min | % | min | ||
| mid1-mYFP | ND | ND | 17.1 ± 7.4 (4)/27.7 ± 10 (23) | −11.2 ± 1.1 (9) |
| mid1-mECitrine | 3.03 ± 1.32 (10) | 68.6 | ND | ND |
| mid1-mECitrine cdr2Δ | 1.97 ± 0.77 (10) | 57.8 | ND | ND |
| mYFP-rng2 | 1.77 ± 1.07 (14) | 75.4 | 9.4 ± 5.6 (25)/28.0 ± 16.9 (25) | −12.5 ± 1.1 (8) |
| mYFP-rng2 myo2-IQ1ΔIQ2Δ | 1.39 ± 0.82 (10) | 56.7 | 10.3 ± 3.4 (16)/30.3 ± 9.4 (16)a | −12.8 ± 1.0 (16)a |
| mYFP-rng2 cdr2Δ | 1.29 ± 0.69 (14) | 57.0 | ND | ND |
| mYFP-cdc4 | 0.20 ± 0.14/1.45 ± 0.45 (11) | 21.1/22.3 | 134.1 ± 93.9 (29)/286.3 ± 130.4 (29) | −11.9 ± 1.4 (11) |
| mYFP-cdc4 myo2-IQ1ΔIQ2Δ | NA/1.39 ± 0.89 (24) | NA/53.4 | 107.5 ± 59.7 (16)/265.0 ± 90.1 (16)a | −11.4 ± 1.5 (16)a |
| mYFP-myo2 | 0.51 ± 0.22 (14) | 59.5 | 55.3 ± 32.8 (31) | −10.2 ± 2.5 (9) |
| mYFP-myo2-IQ1ΔIQ2Δ | 0.48 ± 0.31 (18) | 71.4 | ND | ND |
| mYFP-myo2 cdr2Δ | 0.52 ± 0.22 (11) | 79.7 | ND | ND |
| rlc1-mYFP | 0.51 ± 0.22 (18) | 61.9 | 41.3 ± 23 (31) | −10.2 ± 1.8 (14) |
| mYFP-cdc15 | 0.07 ± 0.02/1.10 ± 0.67 (15) | 57.3/27.3 | 36.3 ± 16.9 (21) | −4.9 ± 0.6 (12) |
| mYFP-cdc15 cdr2Δ | 0.08 ± 0.04/0.83 ± 0.62 (15) | 50.9/49.1 | ND | ND |
| cdc12-3YFPb | 0.50 ± 0.17 (11) | 69.3 | 6.9 ± 2.9 (16) | 1.0 ± 0.6 (16) |