Localization under test conditions. Data is given as node, ring, and clump, respectively. Except for rlc1, both null and temperature-sensitive mutants were tested, and the same results were obtained. Italic +/− indicates the genetic dependencies tested in this study; non-italic +/− indicates the published genetic results not tested in this study. Because our experiments focus on the node assembly, the published genetic dependencies on ring and clump formation were not cited here.

Wu et al., 2006.

Wu et al., 2003.

Lat-A treatment.

In the absence of Mid1, cytokinesis nodes do not form. After a delay, cells usually assemble a contractile ring that is generally mislocalized and constricts slowly (Wu et al., 2003; Hachet and Simanis, 2008; Huang et al., 2008).

Sohrmann et al., 1996; Bähler et al., 1998a.

Paoletti and Chang, 2000.

Motegi et al., 2000: no Myo2 signal was detected in the cdc4 temperature-sensitive strain. But in our study, we show that Myo2 localizes as a faint disorganized structure.

Mulvihill et al., 2001.

Le Goff et al., 2000.

Huang et al., 2008.

Naqvi et al., 2000.

Hachet and Simanis, 2008.

Coffman et al., 2009.