Table I.

Percentage of CHO cells with granules containing the indicated P-granule components

P-granule component Localization to P granules Motifs aFormation of amorphous aggregates bFormation of globular granules cColocalization with PGL-3 granules 
   % % % 
PGL-3 Constitutive RGG box 4 96 N.A. 
PGL-1 Constitutive RGG box 10 82 100 
GLH-1 Constitutive DEAD box, 4 CCHC fingers 2 0 4 
GLH-3 Constitutive DEAD box, 2 CCHC fingers 4 0 20 
MEX-1 Transient 2 CCCH fingers 20 0 100 
MEX-3 Transient 2 KH domains 4 0 92 
POS-1 Transient 2 CCCH fingers 50 0 82 
OMA-1 Transient 2 CCCH fingers 8 0 90 
OMA-2 Transient 2 CCCH fingers 10 0 92 
SPN-4 Transient RNP motif 8 0 98 
GLD-1 Transient KH domain 10 0 92 
CGH-1 Transient DEAD box 16 0 80 
GLD-3 Transient KH-related domains 4 0 16 
IFE-1 Transient Translation initiation factor 4E 0 0 6 
a

Percentage of cells in which the expressed GST-tagged P-granule components formed amorphous aggregates.

b

Percentage of cells in which the expressed GST-tagged P-granule components formed globular granules.

c

Percentage of cells in which the expressed GST-tagged P-granule components colocalized with the granules formed by 6xHis-tagged PGL-3.

50 cells were randomly selected in each expression experiment, and the number of the cells forming granules was counted. In the coexpression experiments, we counted cells with granules that contained both PGL-3 and the corresponding component. N.A., not applicable.

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