Percentage of CHO cells with granules containing the indicated P-granule components
| P-granule component | Localization to P granules | Motifs | aFormation of amorphous aggregates | bFormation of globular granules | cColocalization with PGL-3 granules |
| % | % | % | |||
| PGL-3 | Constitutive | RGG box | 4 | 96 | N.A. |
| PGL-1 | Constitutive | RGG box | 10 | 82 | 100 |
| GLH-1 | Constitutive | DEAD box, 4 CCHC fingers | 2 | 0 | 4 |
| GLH-3 | Constitutive | DEAD box, 2 CCHC fingers | 4 | 0 | 20 |
| MEX-1 | Transient | 2 CCCH fingers | 20 | 0 | 100 |
| MEX-3 | Transient | 2 KH domains | 4 | 0 | 92 |
| POS-1 | Transient | 2 CCCH fingers | 50 | 0 | 82 |
| OMA-1 | Transient | 2 CCCH fingers | 8 | 0 | 90 |
| OMA-2 | Transient | 2 CCCH fingers | 10 | 0 | 92 |
| SPN-4 | Transient | RNP motif | 8 | 0 | 98 |
| GLD-1 | Transient | KH domain | 10 | 0 | 92 |
| CGH-1 | Transient | DEAD box | 16 | 0 | 80 |
| GLD-3 | Transient | KH-related domains | 4 | 0 | 16 |
| IFE-1 | Transient | Translation initiation factor 4E | 0 | 0 | 6 |
| P-granule component | Localization to P granules | Motifs | aFormation of amorphous aggregates | bFormation of globular granules | cColocalization with PGL-3 granules |
| % | % | % | |||
| PGL-3 | Constitutive | RGG box | 4 | 96 | N.A. |
| PGL-1 | Constitutive | RGG box | 10 | 82 | 100 |
| GLH-1 | Constitutive | DEAD box, 4 CCHC fingers | 2 | 0 | 4 |
| GLH-3 | Constitutive | DEAD box, 2 CCHC fingers | 4 | 0 | 20 |
| MEX-1 | Transient | 2 CCCH fingers | 20 | 0 | 100 |
| MEX-3 | Transient | 2 KH domains | 4 | 0 | 92 |
| POS-1 | Transient | 2 CCCH fingers | 50 | 0 | 82 |
| OMA-1 | Transient | 2 CCCH fingers | 8 | 0 | 90 |
| OMA-2 | Transient | 2 CCCH fingers | 10 | 0 | 92 |
| SPN-4 | Transient | RNP motif | 8 | 0 | 98 |
| GLD-1 | Transient | KH domain | 10 | 0 | 92 |
| CGH-1 | Transient | DEAD box | 16 | 0 | 80 |
| GLD-3 | Transient | KH-related domains | 4 | 0 | 16 |
| IFE-1 | Transient | Translation initiation factor 4E | 0 | 0 | 6 |
Percentage of cells in which the expressed GST-tagged P-granule components formed amorphous aggregates.
Percentage of cells in which the expressed GST-tagged P-granule components formed globular granules.
Percentage of cells in which the expressed GST-tagged P-granule components colocalized with the granules formed by 6xHis-tagged PGL-3.
50 cells were randomly selected in each expression experiment, and the number of the cells forming granules was counted. In the coexpression experiments, we counted cells with granules that contained both PGL-3 and the corresponding component. N.A., not applicable.