Reevaluation of Tat components in pea chloroplasts and thylakoids
| Protein | Intact chloroplasts (molecule/cp) | Thylakoids (molecule/thylakoid) | Stoichiometry | |||
| Mean | SD | Mean | SD | Chloroplasts | Thylakoids | |
| cpTatC | 8,800 | 400 | 9,700 | 200 | 1 | 1 |
| Hcf106 | 39,000 | 2,000 | 37,700 | 1,800 | ∼4 | ∼4 |
| Tha4 | 186,500 | 12,900 | 127,900 | 31,700 | ∼21 | ∼13 |
| Protein | Intact chloroplasts (molecule/cp) | Thylakoids (molecule/thylakoid) | Stoichiometry | |||
| Mean | SD | Mean | SD | Chloroplasts | Thylakoids | |
| cpTatC | 8,800 | 400 | 9,700 | 200 | 1 | 1 |
| Hcf106 | 39,000 | 2,000 | 37,700 | 1,800 | ∼4 | ∼4 |
| Tha4 | 186,500 | 12,900 | 127,900 | 31,700 | ∼21 | ∼13 |
The molecules of cpTat components per chloroplast and thylakoid were determined by quantitative immunoblotting using full-length IVT protein standards (see Materials and methods, Fig. S1, and Tables S1 and S2) and are the means and SDs of three biological replicates. Chloroplasts and washed thylakoids were isolated from pea seedlings (see Materials and methods).