Fluorescence intensities of IR-induced nuclear foci
| Treatment | 53BP1 | γ-H2AX | ||||
| Number of cells (n) | Foci per nucleus | Fluorescence intensitya | Number of cells (n) | Foci per nucleus | Fluorescence intensitya | |
| AU | AU | |||||
| Control siRNA | 2,377 | 26 | 4,100 | 2,501 | 22 | 1,588 |
| RNF169 siRNA | 1,351 | 25 | 6,127 (1.49×)b | 1,343 | 19 | 1,685 (1.06×)b |
| Treatment | 53BP1 | γ-H2AX | ||||
| Number of cells (n) | Foci per nucleus | Fluorescence intensitya | Number of cells (n) | Foci per nucleus | Fluorescence intensitya | |
| AU | AU | |||||
| Control siRNA | 2,377 | 26 | 4,100 | 2,501 | 22 | 1,588 |
| RNF169 siRNA | 1,351 | 25 | 6,127 (1.49×)b | 1,343 | 19 | 1,685 (1.06×)b |
U2OS cells treated with control or RNF169 siRNAs for 3 d were exposed to IR (0.25 Gy), fixed 30 min later, and immunostained with antibodies to 53BP1 or γ-H2AX. Numbers and intensity of IR-induced nuclear foci were quantified using ScanR image analysis software. Results from a representative experiment are shown. AU, arbitrary unit.
The mean values of total fluorescence associated with all detectable IR-induced nuclear foci.
Fold increase.