Comparative gold grain densities in subcellular compartments devoid of cluster-labeled organelles
| Anti-BDNF immunogold | Anti–pro-BDNF immunogold | |||||||
| Subcellular profile | WT/Myc | cbdnf ko | CON | Bsn | WT/Myc | cbdnf ko | CON | Bsn |
| n = 3 | n = 3 | n = 4 | n = 4 | n = 3 | n = 3 | n = 4 | n = 4 | |
| Dendritea | 3.6 ± 0.5 | 4.7 ± 0.9 | 6.9 ± 0.9 | 6.6 ± 0.7 | 4.7 ± 1.6 | 4.1 ± 1.5 | 6.3 ± 0.3 | 5.1 ± 0.1 |
| Spine | 19.3 ± 2.1 | 18.4 ± 3.8 | 15.0 ± 1.4 | 13.1 ± 0.9 | 18.2 ± 1.2 | 20.3 ± 4.5 | 13.8 ± 0.6 | 14.3 ± 1.6 |
| Myelinated axon | 9.6 ± 1.8 | 11.6 ± 0.7 | 8.3 ± 0.5 | 8.1 ± 1.2 | 12.2 ± 0.8 | 10.7 ± 0.7 | 8.5 ± 1.1 | 10.1 ± 1.5 |
| Anti-BDNF immunogold | Anti–pro-BDNF immunogold | |||||||
| Subcellular profile | WT/Myc | cbdnf ko | CON | Bsn | WT/Myc | cbdnf ko | CON | Bsn |
| n = 3 | n = 3 | n = 4 | n = 4 | n = 3 | n = 3 | n = 4 | n = 4 | |
| Dendritea | 3.6 ± 0.5 | 4.7 ± 0.9 | 6.9 ± 0.9 | 6.6 ± 0.7 | 4.7 ± 1.6 | 4.1 ± 1.5 | 6.3 ± 0.3 | 5.1 ± 0.1 |
| Spine | 19.3 ± 2.1 | 18.4 ± 3.8 | 15.0 ± 1.4 | 13.1 ± 0.9 | 18.2 ± 1.2 | 20.3 ± 4.5 | 13.8 ± 0.6 | 14.3 ± 1.6 |
| Myelinated axon | 9.6 ± 1.8 | 11.6 ± 0.7 | 8.3 ± 0.5 | 8.1 ± 1.2 | 12.2 ± 0.8 | 10.7 ± 0.7 | 8.5 ± 1.1 | 10.1 ± 1.5 |
Two-sided t tests for all profiles in both anti-BDNF immunogold and anti–pro-BDNF immunogold were P > 0.05. All values expressed as the group mean value ± SEM n value correspond to the number of animals examined per experiment. Subcellular profiles are represented as no. of grains/µm2.
Gold grain densities in dendrites are also presented in graph form in Fig. 6 (WT/Myc vs. cbdnf ko) and Fig. 9 (CON vs. Bsn mutant).