Influence of DGK-α on total DAG and PA lipid content
| Sample | Total DAG | Total PA |
| nmol | nmol | |
| si-con | 253.4 ± 24.7 | 1,382.5 ± 42.5 |
| si-DGK-α | 282.5 ± 5.3 | 1,308.5 ± 82.6 |
| R59022 | 197.4 ± 16.5 | 1,365.4 ± 126.9 |
| Sample | Total DAG | Total PA |
| nmol | nmol | |
| si-con | 253.4 ± 24.7 | 1,382.5 ± 42.5 |
| si-DGK-α | 282.5 ± 5.3 | 1,308.5 ± 82.6 |
| R59022 | 197.4 ± 16.5 | 1,365.4 ± 126.9 |
The lipids were separated and analyzed by HPLC mass spectrometry, and the total amount of each DAG and PA in each sample was determined. si-con, siRNA control; si-DGK-α, siRNA targeting DGK-α.