In situ hybridization analysis of E5.5 embryos from Furin+/−;Pace4+/− intercrosses
| Marker | Number of embryos | Normal expression | Reduced expression | Loss of expression |
| Nodal | 61 | 57 | 4 | 0 |
| Fgf8 | 16 | 13 | 3 | 0 |
| Lefty-1/-2 | 14 | 14 | 0 | 0 |
| Lim1 | 9 | 9 | 0 | 0 |
| Fgf5 | 27 | 22 | 3 | 2 |
| Foxd3 | 18 | 18 | 0 | 0 |
| Cer-l | 21 | 21 | 0 | 0 |
| Oct4 | 20 | 20 | 0 | 0 |
| Marker | Number of embryos | Normal expression | Reduced expression | Loss of expression |
| Nodal | 61 | 57 | 4 | 0 |
| Fgf8 | 16 | 13 | 3 | 0 |
| Lefty-1/-2 | 14 | 14 | 0 | 0 |
| Lim1 | 9 | 9 | 0 | 0 |
| Fgf5 | 27 | 22 | 3 | 2 |
| Foxd3 | 18 | 18 | 0 | 0 |
| Cer-l | 21 | 21 | 0 | 0 |
| Oct4 | 20 | 20 | 0 | 0 |
E5.5 embryos from Furin+/−;Pace4+/− intercrosses were stained by whole mount in situ hybridization for markers that are down-regulated at this stage in Nodal mutants (Ben-Haim et al., 2006; Mesnard et al., 2006).