Table II.

pS850 fluorescence intensity values at prometaphase kinetochores

Treatment CHOWT cells CHOS331A cells P-values 
 Fluorescence intensity (pS850/GFP) n (kinetochore pairs, cells) Fluorescence intensity (pS850/GFP) n (kinetochore pairs, cells)  
Taxol 0.81 ± 0.19 89, 10 0.18 ± 0.11 81, 10 P < 0.01 
Nocodazole 0.88 ± 0.33 88, 11 0.12 ± 0.10 74, 10 P < 0.01 

Mean fluorescence intensity values are normalized to GFP staining, and n represents the number of kinetochore pairs quantified followed by the number of cells analyzed. The p-values were calculated using the Student’s t test.

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