pS331 fluorescence intensity values at prometaphase kinetochores
| Treatment | BE control | BE siChk1 | P-values | ||
| Fluorescence intensity (pS331/CENP-A) | n (kinetochore pairs, cells) | Fluorescence intensity (pS331/CENP-A) | n (kinetochore pairs, cells) | ||
| Unperturbed | 1.15 ± 0.52 | 196, 12 | 0.10 ± 0.07 | 80, 12 | P < 0.01 |
| Taxol | 0.87 ± 0.43 | 188, 12 | 0.08 ± 0.06 | 174, 12 | P < 0.01 |
| Nocodazole | 0.88 ± 0.45 | 162, 12 | 0.92 ± 0.44 | 193, 12 | P = 0.76 |
| Treatment | BE control | BE siChk1 | P-values | ||
| Fluorescence intensity (pS331/CENP-A) | n (kinetochore pairs, cells) | Fluorescence intensity (pS331/CENP-A) | n (kinetochore pairs, cells) | ||
| Unperturbed | 1.15 ± 0.52 | 196, 12 | 0.10 ± 0.07 | 80, 12 | P < 0.01 |
| Taxol | 0.87 ± 0.43 | 188, 12 | 0.08 ± 0.06 | 174, 12 | P < 0.01 |
| Nocodazole | 0.88 ± 0.45 | 162, 12 | 0.92 ± 0.44 | 193, 12 | P = 0.76 |
Mean fluorescence intensity values are normalized to CENP-A staining, and n represents the number of kinetochore pairs quantified followed by the number of cells analyzed. The p-values were calculated using the Student’s t test.