DSB repair defects in tefu and mei-41 mutants
| Genotype | Mean γ-H2AV foci per oocyte | |
| Region 2a | Region 3 | |
| Wild type | 6.2 ± 1.1 | 0.1 ± 0.3 |
| spn-A1 | 3.7 ± 1.4a | 22.8 ± 3.4 |
| 25.2 ± 3.6b | ||
| tefu8 | ND | 39.1 ± 8.0b |
| mei-41D3 | 4.2 ± 0.8a | 21.0 ± 1.3 |
| Wild-type nurse cells | 3.6 ± 1.6 | 0.0 |
| tefu8 nurse cells | 9.3 ± 2.8 | 0.0 |
| mei-W684572;tefu8 | 0.0 | 0.0 |
| mei-41D3;tefu8, 18° | 7.5 ± 1.7 | 18.2 ± 2.1 |
| mei-41D3;tefu8, 25° | 0.0 | 0.0 |
| mei-41D3;tefu8, 25–18° | 7.3 ± 2.6 | 17.5 ± 3.3 |
| Genotype | Mean γ-H2AV foci per oocyte | |
| Region 2a | Region 3 | |
| Wild type | 6.2 ± 1.1 | 0.1 ± 0.3 |
| 3.7 ± 1.4 | 22.8 ± 3.4 | |
| 25.2 ± 3.6 | ||
| ND | 39.1 ± 8.0 | |
| 4.2 ± 0.8 | 21.0 ± 1.3 | |
| Wild-type nurse cells | 3.6 ± 1.6 | 0.0 |
| 9.3 ± 2.8 | 0.0 | |
| 0.0 | 0.0 | |
| 7.5 ± 1.7 | 18.2 ± 2.1 | |
| 0.0 | 0.0 | |
| 7.3 ± 2.6 | 17.5 ± 3.3 | |
Foci were manually counted, except as otherwise noted. Means ± SD are shown.
Mutations in DSB repair genes cause a delay in γ-H2AV appearance as a result of the activation of the pachytene checkpoint (Joyce and McKim, 2009).
The foci number is an estimate based on fluorescent intensity (see Materials and methods) in spn-A1 and tefu8 mutants in which the foci could not be counted because of nearly ubiquitous threadlike staining.
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