Ratio measurement values of kinetochore protein copy numbers in S. pombe during G2/M based on our measurements for ScCse4-GFP B
| Protein name | Average number per kinetochore | Average number per kMT |
| cnp1 | 15.1 ± 3.4 | 5.0 ± 1.1 |
| mif2 | 11.1 ± 3.1 | 3.7 ± 1.0 |
| mal2 | 32.3 ± 6.3 | 10.8 ± 2.1 |
| sim4 | 36.2 ± 8.5 | 12.1 ± 2.8 |
| fta1 | 11.1 | 3.7 |
| fta2 | 29 | 9.7 |
| fta3 | 36.8 ± 7.2 | 12.3 ± 2.4 |
| mis12 | 46.7 ± 9.9 | 15.6 ± 3.3 |
| spc7 | 34.8 ± 7.0 | 11.6 ± 2.3 |
| ndc80 | 66.1 ± 14.0 | 22.0 ± 4.7 |
| dad1 | 10 | 3.3 |
| Protein name | Average number per kinetochore | Average number per kMT |
| cnp1 | 15.1 ± 3.4 | 5.0 ± 1.1 |
| mif2 | 11.1 ± 3.1 | 3.7 ± 1.0 |
| mal2 | 32.3 ± 6.3 | 10.8 ± 2.1 |
| sim4 | 36.2 ± 8.5 | 12.1 ± 2.8 |
| fta1 | 11.1 | 3.7 |
| fta2 | 29 | 9.7 |
| fta3 | 36.8 ± 7.2 | 12.3 ± 2.4 |
| mis12 | 46.7 ± 9.9 | 15.6 ± 3.3 |
| spc7 | 34.8 ± 7.0 | 11.6 ± 2.3 |
| ndc80 | 66.1 ± 14.0 | 22.0 ± 4.7 |
| dad1 | 10 | 3.3 |
S. pombe copy numbers per kinetochore were obtained by multiplying the fluorescence ratio of ScCse4-GFP B to each S. pombe GFP fusion protein (Joglekar et al., 2008) by the number of ScCse4-GFP B molecules in an anaphase cluster, 97 ± 19, and dividing by 6, the number of chromosomes in S. pombe. The average copy number per kinetochore was divided by the mean number of microtubule attachments per kinetochore to obtain the average protein copy numbers per kMT in S. pombe (3; Joglekar et al., 2008).