Newly measured values and previously reported ratio measurement values of kinetochore protein copy numbers in S. cerevisiae during anaphase based on our measurements for ScCse4-GFP B
| Protein name | Reported anaphase ratio | Average number per kinetochore |
| Cse4p-Aa | NA | 3.5 ± 0.7 |
| Cse4p-Ba | NA | 6.0 ± 1.2 |
| Cse4p Cir+ a | NA | 4.9 ± 0.9 |
| Cse4p Cir0 a | NA | 5.2 ± 0.9 |
| Mif2pa,b | 0.6 ± 0.1 | 3.6 ± 0.8 |
| Ctf3p | 0.5 ± 0.01 | 3.0 ± 0.6 |
| Ndc10pa | 1.2 ± 0.2 | 7.4 ± 1.6 |
| Cep3pa | 0.6 ± 0.1 | 3.4 ± 0.8 |
| Ctf19pb | 3.4 ± 0.2 | 6.4 ± 1.3 |
| Chl4p | 0.3 ± 0.01 | 1.6 ± 0.3 |
| Nkp2pb | 6.1 ± 0.1 | 3.6 ± 0.7 |
| Mtw1p | 2.4 ± 0.1 | 14.5 ± 2.9 |
| Spc105p | 2.4 ± 0.01 | 14.5 ± 2.8 |
| Nuf2p | 3.6 ± 0.2 | 21.7 ± 4.4 |
| Ndc80pa | 3.2 ± 0.5 | 19.1 ± 3.9 |
| Ask1p | 5.3 ± 0.3 | 32.0 ± 6.5 |
| Scm3pa | 0.23 ± 0.05 | 1.3 ± 0.3 |
| Scm3pac | NA | BG |
| Protein name | Reported anaphase ratio | Average number per kinetochore |
| Cse4p-Aa | NA | 3.5 ± 0.7 |
| Cse4p-Ba | NA | 6.0 ± 1.2 |
| Cse4p Cir+ a | NA | 4.9 ± 0.9 |
| Cse4p Cir0 a | NA | 5.2 ± 0.9 |
| Mif2pa,b | 0.6 ± 0.1 | 3.6 ± 0.8 |
| Ctf3p | 0.5 ± 0.01 | 3.0 ± 0.6 |
| Ndc10pa | 1.2 ± 0.2 | 7.4 ± 1.6 |
| Cep3pa | 0.6 ± 0.1 | 3.4 ± 0.8 |
| Ctf19pb | 3.4 ± 0.2 | 6.4 ± 1.3 |
| Chl4p | 0.3 ± 0.01 | 1.6 ± 0.3 |
| Nkp2pb | 6.1 ± 0.1 | 3.6 ± 0.7 |
| Mtw1p | 2.4 ± 0.1 | 14.5 ± 2.9 |
| Spc105p | 2.4 ± 0.01 | 14.5 ± 2.8 |
| Nuf2p | 3.6 ± 0.2 | 21.7 ± 4.4 |
| Ndc80pa | 3.2 ± 0.5 | 19.1 ± 3.9 |
| Ask1p | 5.3 ± 0.3 | 32.0 ± 6.5 |
| Scm3pa | 0.23 ± 0.05 | 1.3 ± 0.3 |
| Scm3pac | NA | BG |
For the kinetochore proteins not measured in this study, we assumed the same ratio of fluorescence relative to Cse4-GFP fluorescence in the yeast Cse4-GFP B used as the standard in previous studies (Joglekar et al., 2006, 2008; Johnston et al., 2010). Average protein copy numbers per kinetochore for S. cerevisiae were obtained by multiplying the average number of ScCse4-GFP B per kinetochore, 6.0 ± 1.2, by the fluorescence ratio of ScCse4-GFP B to each GFP-fusion protein (Joglekar et al., 2006). Ratios and average number per kinetochore values indicated by the footnotes were calculated using our own measurements. Ratios reported in Joglekar et al. (2006) as ratios to ScNuf2 signal were converted to ScCse4 B ratios before the multiplication of the average number of ScCse4-GFP B per kinetochore, 6.0 ± 1.2.
Our measured value.
Reported ratio is calculated as (Nuf2 signal):(protein signal) as it is presented in Joglekar et al. (2006).
Azide deoxyglucose treatment used.