Calculated parameters derived from FRAP measurements in BAC HeLa and E3 U2-OS cell lines
| Proteins | Nucleoplasm | Splicing factor compartments | E3 gene loci | ||||
| Df DRB | koff nt | koff isog | Df isog | Df DRB | kofwnt | koff E3 gene loci | |
| µm2s−1 | s−1 | s−1 | µm2s−1 | µm2s−1 | s−1 | s−1 | |
| U1-70Kab | 0.27 ± 0.02 | 1.57 ± 0.21 | 0.058 ± 0.007 | NA | 0.33 ± 0.05 | 1.07 ± 0.26 | NA |
| U2A′ab | 0.56 ± 0.05 | 0.062 ± 0.005 | 0.047 ± 0.006 | NA | 0.17 ± 0.02 | 0.050 ± 0.08 | NA |
| hPrp31ab | 0.55 ± 0.03 | 1.36 ± 0.30 | 0.63 ± 0.26 | NA | 0.24 ± 0.03 | 0.055 ± 0.005 | NA |
| hPrp4ab | 0.47 ± 0.08 | 1.55 ± 0.13 | 0.90 ± 0.23 | NA | 0.32 ± 0.04 | 0.048 ± 0.007 | NA |
| hPrp8ab | 0.27 ± 0.02 | 0.037 ± 0.004 | NA | 0.40 ± 0.02 | 0.10 ± 0.04 | 0.035 ± 0.005 | NA |
| Snu114ab | 0.26 ± 0.03 | 0.032 ± 0.006 | NA | 0.27 ± 0.03 | 0.19 ± 0.02 | 0.038 ± 0.007 | NA |
| U1-70Kac | 0.50 ± 0.05 | 1.88 ± 0.15 | ND | ND | ND | ND | 0.112 ± 0.010 |
| U2B″c | 0.61 ± 0.07 | 0.064 ± 0.005 | ND | ND | ND | ND | 0.056 ± 0.009 |
| hPrp4c | 0.75 ± 0.08 | 1.51 ± 0.20 | ND | ND | ND | ND | 0.043 ± 0.006 |
| hPrp8ac | 0.37 ± 0.03 | 0.040 ± 0.003 | ND | ND | ND | ND | 0.030 ± 0.002 |
| Proteins | Nucleoplasm | Splicing factor compartments | E3 gene loci | ||||
| Df DRB | koff nt | koff isog | Df isog | Df DRB | kofwnt | koff E3 gene loci | |
| µm2s−1 | s−1 | s−1 | µm2s−1 | µm2s−1 | s−1 | s−1 | |
| U1-70Kab | 0.27 ± 0.02 | 1.57 ± 0.21 | 0.058 ± 0.007 | NA | 0.33 ± 0.05 | 1.07 ± 0.26 | NA |
| U2A′ab | 0.56 ± 0.05 | 0.062 ± 0.005 | 0.047 ± 0.006 | NA | 0.17 ± 0.02 | 0.050 ± 0.08 | NA |
| hPrp31ab | 0.55 ± 0.03 | 1.36 ± 0.30 | 0.63 ± 0.26 | NA | 0.24 ± 0.03 | 0.055 ± 0.005 | NA |
| hPrp4ab | 0.47 ± 0.08 | 1.55 ± 0.13 | 0.90 ± 0.23 | NA | 0.32 ± 0.04 | 0.048 ± 0.007 | NA |
| hPrp8ab | 0.27 ± 0.02 | 0.037 ± 0.004 | NA | 0.40 ± 0.02 | 0.10 ± 0.04 | 0.035 ± 0.005 | NA |
| Snu114ab | 0.26 ± 0.03 | 0.032 ± 0.006 | NA | 0.27 ± 0.03 | 0.19 ± 0.02 | 0.038 ± 0.007 | NA |
| U1-70Kac | 0.50 ± 0.05 | 1.88 ± 0.15 | ND | ND | ND | ND | 0.112 ± 0.010 |
| U2B″c | 0.61 ± 0.07 | 0.064 ± 0.005 | ND | ND | ND | ND | 0.056 ± 0.009 |
| hPrp4c | 0.75 ± 0.08 | 1.51 ± 0.20 | ND | ND | ND | ND | 0.043 ± 0.006 |
| hPrp8ac | 0.37 ± 0.03 | 0.040 ± 0.003 | ND | ND | ND | ND | 0.030 ± 0.002 |
NA, not applicable. Diffusion coefficients Df FRAP DRB were calculated from fits of the FRAP curves measured in the nucleoplasm and the splicing factor compartments in DRB-treated cells. Dissociation rates koff nt were derived from fits of the FRAP curves measured in the nucleoplasm and splicing factor compartments of nontreated cells. Kinetic parameters koff nucl isog and Df nucl isog were derived from fits of the FRAP curves measured in the nucleoplasm of isoginkgetin-treated cells. Dissociation rates koff E3 gene loci were calculated from fits of the FRAP curves measured at the transcription site of the E3 transgene in doxycycline-treated E3 U2-OS cells. The mean ± SEM is shown.
BAC stable cell line.
HeLa cells.
E3 U2-OS cells.