Dye anisotropies in the ribosomal tunnel and at the membranea
| PMP RTC | Dye location | NBD rb | BOP rc |
| 2TML12DA2122 | Tunnel | 0.31 ± 0.01 | 0.30 ± 0.01 |
| 2TML12DA2125 | Tunnel | 0.30 ± 0.01 | 0.30 ± 0.02 |
| 2TML12DA2126 | Tunnel | 0.28 ± 0.02 | 0.29 ± 0.02 |
| 2TML12DA2130 | Tunnel | 0.29 ± 0.01 | 0.29 ± 0.01 |
| 2TML12DA2FLd | Membranee | 0.27 ± 0.03 | 0.29 ± 0.01 |
| 3TML12,18DA3159 | Tunnel | 0.30 ± 0.03 | 0.28 ± 0.01 |
| 3TML12,18DA3163 | Tunnel | 0.29 ± 0.02 | 0.29 ± 0.01 |
| 3TML12,18DA3167 | Tunnel | 0.29 ± 0.03 | 0.29 ± 0.01 |
| PMP RTC | Dye location | NBD rb | BOP rc |
| 2TML12DA2122 | Tunnel | 0.31 ± 0.01 | 0.30 ± 0.01 |
| 2TML12DA2125 | Tunnel | 0.30 ± 0.01 | 0.30 ± 0.02 |
| 2TML12DA2126 | Tunnel | 0.28 ± 0.02 | 0.29 ± 0.02 |
| 2TML12DA2130 | Tunnel | 0.29 ± 0.01 | 0.29 ± 0.01 |
| 2TML12DA2FLd | Membranee | 0.27 ± 0.03 | 0.29 ± 0.01 |
| 3TML12,18DA3159 | Tunnel | 0.30 ± 0.03 | 0.28 ± 0.01 |
| 3TML12,18DA3163 | Tunnel | 0.29 ± 0.02 | 0.29 ± 0.01 |
| 3TML12,18DA3167 | Tunnel | 0.29 ± 0.03 | 0.29 ± 0.01 |
Data are the average ± SD for three or more independent experiments.
NBD: λex = 468 nm, λem = 530 nm, bandpass = 4 nm; measured in absence of acceptor.
BOP, λex = 575 nm, λem = 595 nm, bandpass = 4 nm; measured in absence of donor.
Full-length 2TML12DA2 proteins were translated, released from the translocon, and integrated into the ER membrane.
Dyes are at opposite surfaces of ER membrane, with donor in lumen and acceptor in cytosol.