Collisional quenching of integration intermediates with two nascent chain TMSsa
| PMPb | TMSC-PMP separationc | nd | −MLT Ksve | +MLT Ksve | ΔKsv |
| M−1 ± SD | M−1 ± SD | M−1 | |||
| 2TML53K2163 | 3 | 4 | 3.1 ± 0.2 | 3.4 ± 0.3 | 0.3 ± 0.4 |
| 2TML53K2166 | 6 | 3 | 1.8 ± 0.3 | 4.0 ± 0.1 | 2.2 ± 0.3 |
| 2TML53K2171 | 11 | 6 | 2.2 ± 0.2 | 4.2 ± 0.2 | 2.0 ± 0.3 |
| 2TML53K2171 (+PK, RNase) | 11 | 6 | 0.6 ± 0.4 | 3.2 ± 0.2 | 2.6 ± 0.4 |
| 2TML12K2122 | 3 | 3 | 2.8 ± 0.1 | 3.2 ± 0.2 | 0.4 ± 0.2 |
| 2TML12K2126 | 7 | 3 | 2.0 ± 0.2 | 4.0 ± 0.3 | 2.0 ± 0.4 |
| 2TML12K2130 | 11 | 3 | 1.9 ± 0.1 | 3.9 ± 0.2 | 2.0 ± 0.2 |
| 2TML12K2148 | 29 | 3 | 1.7 ± 0.1 | 4.2 ± 0.3 | 2.5 ± 0.3 |
| 2TML12KN122 | 3 | 2 | 2.0 ± 0.5 | 4.6 ± 0.2 | 2.6 ± 0.5 |
| 1.5TML53K1.5163 | 15 | 2 | 3.4 ± 0.0 | 3.6 ± 0.1 | 0.2 ± 0.1 |
| 1.5TML53K1.5171 | 23 | 2 | 3.6 ± 0.3 | 3.8 ± 0.1 | 0.2 ± 0.3 |
| 2DUPL54K2159 | 3 | 2 | 4.0 ± 0.2 | 4.1 ± 0.1 | 0.1 ± 0.2 |
| 2DUPL54K2162 | 6 | 2 | 2.0 ± 0.1 | 4.1 ± 0.1 | 2.1 ± 0.1 |
| 2INVL54K191 | 3 | 2 | 1.1 ± 0.1 | 3.4 ± 0.4 | 2.3 ± 0.4 |
| 2INVL54K194 | 6 | 2 | 3.1 ± 0.4 | 3.4 ± 0.1 | 0.3 ± 0.4 |
| 2INVL54K2163 | 3 | 2 | 4.0 ± 0.1 | 4.0 ± 0.1 | 0.0 ± 0.1 |
| 2INVL54K2166 | 6 | 2 | 1.9 ± 0.0 | 4.2 ± 0.2 | 2.3 ± 0.2 |
| PMPb | TMSC-PMP separationc | nd | −MLT Ksve | +MLT Ksve | ΔKsv |
| M−1 ± SD | M−1 ± SD | M−1 | |||
| 2TML53K2163 | 3 | 4 | 3.1 ± 0.2 | 3.4 ± 0.3 | 0.3 ± 0.4 |
| 2TML53K2166 | 6 | 3 | 1.8 ± 0.3 | 4.0 ± 0.1 | 2.2 ± 0.3 |
| 2TML53K2171 | 11 | 6 | 2.2 ± 0.2 | 4.2 ± 0.2 | 2.0 ± 0.3 |
| 2TML53K2171 (+PK, RNase) | 11 | 6 | 0.6 ± 0.4 | 3.2 ± 0.2 | 2.6 ± 0.4 |
| 2TML12K2122 | 3 | 3 | 2.8 ± 0.1 | 3.2 ± 0.2 | 0.4 ± 0.2 |
| 2TML12K2126 | 7 | 3 | 2.0 ± 0.2 | 4.0 ± 0.3 | 2.0 ± 0.4 |
| 2TML12K2130 | 11 | 3 | 1.9 ± 0.1 | 3.9 ± 0.2 | 2.0 ± 0.2 |
| 2TML12K2148 | 29 | 3 | 1.7 ± 0.1 | 4.2 ± 0.3 | 2.5 ± 0.3 |
| 2TML12KN122 | 3 | 2 | 2.0 ± 0.5 | 4.6 ± 0.2 | 2.6 ± 0.5 |
| 1.5TML53K1.5163 | 15 | 2 | 3.4 ± 0.0 | 3.6 ± 0.1 | 0.2 ± 0.1 |
| 1.5TML53K1.5171 | 23 | 2 | 3.6 ± 0.3 | 3.8 ± 0.1 | 0.2 ± 0.3 |
| 2DUPL54K2159 | 3 | 2 | 4.0 ± 0.2 | 4.1 ± 0.1 | 0.1 ± 0.2 |
| 2DUPL54K2162 | 6 | 2 | 2.0 ± 0.1 | 4.1 ± 0.1 | 2.1 ± 0.1 |
| 2INVL54K191 | 3 | 2 | 1.1 ± 0.1 | 3.4 ± 0.4 | 2.3 ± 0.4 |
| 2INVL54K194 | 6 | 2 | 3.1 ± 0.4 | 3.4 ± 0.1 | 0.3 ± 0.4 |
| 2INVL54K2163 | 3 | 2 | 4.0 ± 0.1 | 4.0 ± 0.1 | 0.0 ± 0.1 |
| 2INVL54K2166 | 6 | 2 | 1.9 ± 0.0 | 4.2 ± 0.2 | 2.3 ± 0.2 |
RTC preparation and spectroscopic analyses are described in Materials and methods.
PMP topogenic sequences and NBD locations are shown in Figs. 2 A and 3 G.
TMSC, C-terminal end of TMS nearest the PTC; TMSC–PTC separation, nascent chain residues between TMSC and the PTC.
n, number of independent experiments.
–MLT and +MLT, data obtained prior to or after, respectively, melittin addition.