Plasmids used in this study
| Plasmid name (ASP) | Construct |
| 451 | pGEX-6P-1 (GE Healthcare) containing a modified MCS for bacterial expression of GST-tagged proteins |
| 1,633 | GST–xM18BP1-2161–415 |
| 1,980 | GST–xM18BP1-1161–375 |
| 1,639 | GST-xHJURP |
| 1,938 | 6His-xHJURP42–194 |
| 447 | pCS2+ containing a modified MCS for IVT of the protein in rabbit reticulocyte lysate or in vitro transcription of RNA |
| 459 | pCS2+ containing 6×myc sequence upstream of the MCS for IVT of the protein in rabbit reticulocyte lysate or in vitro transcription of RNA |
| 1,730 | pCS2+ containing 3×FLAG sequence upstream of the MCS for IVT of the protein in rabbit reticulocyte lysate or in vitro transcription of RNA |
| 1,853 | pCS2+ containing 3×FLAG sequence downstream of the MCS for IVT of protein in rabbit reticulocyte lysate or in vitro transcription of RNA |
| 1,592 | p447-xHJURP for production of xHJURP RNA |
| 1,815 | p447-xHJURP (codon optimized) for production of the xHJURP IVT protein |
| 1,970 | p447-hHJURP (codon optimized) |
| 1,968 | p1853-xHJURP (codon optimized) |
| 1,969 | p1853-hHJURP (codon optimized) |
| 559 | p459–xCENP-A |
| 1,819 | p1730–xCENP-A |
| 1,870 | p1853–xM18BP1-1 |
| 1,871 | p1853–xM18BP1-2 |
| 867 | p459–xCENP-C (codon optimized) |
| 1,330 | p459–xCENP-C amino acids 1–380 (codon optimized) |
| 1,925 | p459–xCENP-C amino acids 200–579 (codon optimized) |
| 1,928 | p459–xCENP-C amino acids 381–711 (codon optimized) |
| 1,926 | p459–xCENP-C amino acids 580–949 (codon optimized) |
| 1,920 | p459–xCENP-C amino acids 712–1,190 (codon optimized) |
| 1,927 | p459–xCENP-C amino acids 950–1,291 (codon optimized) |
| 1,150 | p459–xCENP-C amino acids 1,191–1,400 (codon optimized) |
| 1,943 | p459–xCENP-C amino acids 1,204–1,400 (codon optimized) |
| 1,935 | p459–xCENP-C amino acids 1,229–1,400 (codon optimized) |
| 1,921 | p459–xCENP-C amino acids 1,191–1,350 (codon optimized) |
| 1,936 | p459–xCENP-C amino acids 1,191–1,310 (codon optimized) |
| 866 | p447–xCENP-C (codon optimized) |
| 1,350 | p447–xM18BP1-1 |
| 1,351 | p447–xM18BP1-2 |
| 844 | p447–hCENP-C |
| 1,828 | p447-hM18BP1 (codon optimized) |
| 572 | p447–mCherry-tubulin |
| 1,981 | p459-dTm2 (Drosophila melanogaster tropomyosin 2) |
| Plasmid name (ASP) | Construct |
| 451 | pGEX-6P-1 (GE Healthcare) containing a modified MCS for bacterial expression of GST-tagged proteins |
| 1,633 | GST–xM18BP1-2161–415 |
| 1,980 | GST–xM18BP1-1161–375 |
| 1,639 | GST-xHJURP |
| 1,938 | 6His-xHJURP42–194 |
| 447 | pCS2+ containing a modified MCS for IVT of the protein in rabbit reticulocyte lysate or in vitro transcription of RNA |
| 459 | pCS2+ containing 6×myc sequence upstream of the MCS for IVT of the protein in rabbit reticulocyte lysate or in vitro transcription of RNA |
| 1,730 | pCS2+ containing 3×FLAG sequence upstream of the MCS for IVT of the protein in rabbit reticulocyte lysate or in vitro transcription of RNA |
| 1,853 | pCS2+ containing 3×FLAG sequence downstream of the MCS for IVT of protein in rabbit reticulocyte lysate or in vitro transcription of RNA |
| 1,592 | p447-xHJURP for production of xHJURP RNA |
| 1,815 | p447-xHJURP (codon optimized) for production of the xHJURP IVT protein |
| 1,970 | p447-hHJURP (codon optimized) |
| 1,968 | p1853-xHJURP (codon optimized) |
| 1,969 | p1853-hHJURP (codon optimized) |
| 559 | p459–xCENP-A |
| 1,819 | p1730–xCENP-A |
| 1,870 | p1853–xM18BP1-1 |
| 1,871 | p1853–xM18BP1-2 |
| 867 | p459–xCENP-C (codon optimized) |
| 1,330 | p459–xCENP-C amino acids 1–380 (codon optimized) |
| 1,925 | p459–xCENP-C amino acids 200–579 (codon optimized) |
| 1,928 | p459–xCENP-C amino acids 381–711 (codon optimized) |
| 1,926 | p459–xCENP-C amino acids 580–949 (codon optimized) |
| 1,920 | p459–xCENP-C amino acids 712–1,190 (codon optimized) |
| 1,927 | p459–xCENP-C amino acids 950–1,291 (codon optimized) |
| 1,150 | p459–xCENP-C amino acids 1,191–1,400 (codon optimized) |
| 1,943 | p459–xCENP-C amino acids 1,204–1,400 (codon optimized) |
| 1,935 | p459–xCENP-C amino acids 1,229–1,400 (codon optimized) |
| 1,921 | p459–xCENP-C amino acids 1,191–1,350 (codon optimized) |
| 1,936 | p459–xCENP-C amino acids 1,191–1,310 (codon optimized) |
| 866 | p447–xCENP-C (codon optimized) |
| 1,350 | p447–xM18BP1-1 |
| 1,351 | p447–xM18BP1-2 |
| 844 | p447–hCENP-C |
| 1,828 | p447-hM18BP1 (codon optimized) |
| 572 | p447–mCherry-tubulin |
| 1,981 | p459-dTm2 (Drosophila melanogaster tropomyosin 2) |
ASP, A.F. Straight plasmid.