Characteristics of Arf6 mutant proteins
| Arf6 mutant protein | Phenotype | Citation | VSVG sortinga |
| Arf6Q67L | Locked in GTP form | Donaldson, 2003 | Apical |
| Arf6D125N | Binds xanthine nucleotides, presumed nucleotide-free in cells | Yang and Mueckler, 1999 | Apical |
| Arf6T27N | Locked in GDP form | Macia et al., 2004 | Basolateral |
| Arf6N48I | Deficient in PLD activation | Vitale et al., 2005; Jovanovic et al., 2006 | Basolateral |
| Arf6Q67L/Q37E/S38I | Defective in actin remodeling | Al-Awar et al., 2000; Palacios et al., 2001 | Apical |
| Arf6G2A | Can no longer be myristoylated | Knorr et al., 2000 | ND |
| Arf6D125N/G2A | Presumed nucleotide-free, not myristoylated | This paper | Apical |
| Arf6Q67L/G2A | Locked in GTP form, not myristoylated | This paper | Apical |
| Arf6 mutant protein | Phenotype | Citation | VSVG sortinga |
| Arf6Q67L | Locked in GTP form | Donaldson, 2003 | Apical |
| Arf6D125N | Binds xanthine nucleotides, presumed nucleotide-free in cells | Yang and Mueckler, 1999 | Apical |
| Arf6T27N | Locked in GDP form | Macia et al., 2004 | Basolateral |
| Arf6N48I | Deficient in PLD activation | Vitale et al., 2005; Jovanovic et al., 2006 | Basolateral |
| Arf6Q67L/Q37E/S38I | Defective in actin remodeling | Al-Awar et al., 2000; Palacios et al., 2001 | Apical |
| Arf6G2A | Can no longer be myristoylated | Knorr et al., 2000 | ND |
| Arf6D125N/G2A | Presumed nucleotide-free, not myristoylated | This paper | Apical |
| Arf6Q67L/G2A | Locked in GTP form, not myristoylated | This paper | Apical |
The VSVG sorting phenotypes in the presence of Arf6 mutant proteins were all obtained in this study.