Table I. The dynamic behavior of TJ... | Rockefeller University Press

Table I.

The dynamic behavior of TJ components involved in CK2-mediated epithelial barrier regulation

	Epithelium	Pharmacological CK2 inhibition	Mobile fraction (%)
Occludin	Wild type		68 ± 3^†
	Wild type	√	52 ± 5^a
	CK2 knockdown		53 ± 2^a
	CK2 knockdown	√	48 ± 2^a
	ZO-1 knockdown		67 ± 8
	In vivo		57 ± 2^†
	In vivo	√	39 ± 1^a
T404D/S408D	Occludin knockdown		68 ± 4
	Occludin knockdown	√	69 ± 6
T404A/S408A	Occludin knockdown		52 ± 3^a
	Occludin knockdown	√	50 ± 3^a
T404A	Occludin knockdown		68 ± 8
	Occludin knockdown	√	45 ± 4^a
S408A	Occludin knockdown		49 ± 3^a
	Occludin knockdown	√	48 ± 7^a
	ZO-1 knockdown		70 ± 1
S408D	Occludin knockdown		69 ± 3
	Occludin knockdown	√	72 ± 3
	ZO-1 knockdown		66 ± 9
Claudin-1	Occludin knockdown		36 ± 2
	Occludin knockdown/wild-type occludin expression		37 ± 1^†
	Occludin knockdown/S408D occludin expression		35 ± 4
	Occludin knockdown/S408A occludin expression		57 ± 3^a
Claudin-2	Wild type		33 ± 3^†
	Occludin knockdown		33 ± 3
	Occludin knockdown/wild-type occludin expression		31 ± 5
	Occludin knockdown/S408D occludin expression		25 ± 6
	Occludin knockdown/S408A occludin expression		51 ± 2^a
	Wild type	√	51 ± 5
	Occludin knockdown	√	27 ± 1
	ZO-1 knockdown	√	32 ± 2
	ZO-1 knockdown/ZO-1 expression		35 ± 6
	ZO-1 knockdown/ZO-1 expression	√	54 ± 4^a
	ZO-1 knockdown/ZO-1 ΔPDZ1 expression		35 ± 4
	ZO-1 knockdown/ZO-1 ΔPDZ1 expression	√	28 ± 4
	ZO-1 knockdown/ZO-1 ΔU5-Guk expression		33 ± 5
	ZO-1 knockdown/ZO-1 ΔU5-Guk expression	√	32 ± 3
ZO-1	Wild type		48 ± 3^†
	Wild type	√	60 ± 4^a
	Occludin knockdown		46 ± 4
	Occludin knockdown	√	45 ± 4
	Occludin knockdown/wild-type occludin expression		45 ± 4
	Occludin knockdown/S408D occludin expression		43 ± 4
	Occludin knockdown/S408A occludin expression		61 ± 4^a
	Wild-type/large area FRAP (center)		51 ± 4
	Wild-type/large area FRAP (edge)		50 ± 5^†
	Wild-type/large area FRAP (center)	√	46 ± 1
	Wild-type/large area FRAP (edge)	√	58 ± 4^a

	Epithelium	Pharmacological CK2 inhibition	Mobile fraction (%)
Occludin	Wild type		68 ± 3^†
	Wild type	√	52 ± 5^a
	CK2 knockdown		53 ± 2^a
	CK2 knockdown	√	48 ± 2^a
	ZO-1 knockdown		67 ± 8
	In vivo		57 ± 2^†
	In vivo	√	39 ± 1^a
T404D/S408D	Occludin knockdown		68 ± 4
	Occludin knockdown	√	69 ± 6
T404A/S408A	Occludin knockdown		52 ± 3^a
	Occludin knockdown	√	50 ± 3^a
T404A	Occludin knockdown		68 ± 8
	Occludin knockdown	√	45 ± 4^a
S408A	Occludin knockdown		49 ± 3^a
	Occludin knockdown	√	48 ± 7^a
	ZO-1 knockdown		70 ± 1
S408D	Occludin knockdown		69 ± 3
	Occludin knockdown	√	72 ± 3
	ZO-1 knockdown		66 ± 9
Claudin-1	Occludin knockdown		36 ± 2
	Occludin knockdown/wild-type occludin expression		37 ± 1^†
	Occludin knockdown/S408D occludin expression		35 ± 4
	Occludin knockdown/S408A occludin expression		57 ± 3^a
Claudin-2	Wild type		33 ± 3^†
	Occludin knockdown		33 ± 3
	Occludin knockdown/wild-type occludin expression		31 ± 5
	Occludin knockdown/S408D occludin expression		25 ± 6
	Occludin knockdown/S408A occludin expression		51 ± 2^a
	Wild type	√	51 ± 5
	Occludin knockdown	√	27 ± 1
	ZO-1 knockdown	√	32 ± 2
	ZO-1 knockdown/ZO-1 expression		35 ± 6
	ZO-1 knockdown/ZO-1 expression	√	54 ± 4^a
	ZO-1 knockdown/ZO-1 ΔPDZ1 expression		35 ± 4
	ZO-1 knockdown/ZO-1 ΔPDZ1 expression	√	28 ± 4
	ZO-1 knockdown/ZO-1 ΔU5-Guk expression		33 ± 5
	ZO-1 knockdown/ZO-1 ΔU5-Guk expression	√	32 ± 3
ZO-1	Wild type		48 ± 3^†
	Wild type	√	60 ± 4^a
	Occludin knockdown		46 ± 4
	Occludin knockdown	√	45 ± 4
	Occludin knockdown/wild-type occludin expression		45 ± 4
	Occludin knockdown/S408D occludin expression		43 ± 4
	Occludin knockdown/S408A occludin expression		61 ± 4^a
	Wild-type/large area FRAP (center)		51 ± 4
	Wild-type/large area FRAP (edge)		50 ± 5^†
	Wild-type/large area FRAP (center)	√	46 ± 1
	Wild-type/large area FRAP (edge)	√	58 ± 4^a

Data are significant (P < 0.05) relative to control conditions (†).

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