Table I.

cAMP-binding proteins and AKAPs identified from mass spectrometry using different purification methods on HEK 293 cell lysates

Method/cell type Protein description IPI Number of hits Unique peptides 
cAMP resin/untransfected PKA type I-α R-subunit IPI00021831 64 19 
 PKA type II-α R-subunit IPI00219774 104 31 
 PKA type II-β R-subunit IPI00554752 
 AKAP9 IPI00019223 148 70 
 AKAP1 IPI00022585 
cAMP resin/stably transfected TAP-tagged wild-type RIα PKA type I-α R-subunit IPI00021831 57 19 
PKA type II-α R-subunit IPI00219774 15 12 
 PKA type II-β R-subunit IPI00554752 
 AKAP11 IPI00007411 
 AKAP9 IPI00220628 
 AKAP5 IPI00307794 
Streptavidin and calmodulin resins/stably transfected TAP-tagged wild-type RIα PKA type I-α R-subunit IPI00021831 434 29 
PKA α–catalytic subunit IPI00217960 
 PKA α–catalytic subunit IPI00396630 
 AKAP11 IPI00007411 13 
Streptavidin and cAMP resins/stably transfected TAP-tagged wild-type RIα PKA type I-α R-subunit IPI00021831 721 63 
PKA type I-β R-subunit IPI00787996 21 14 
 AKAP11 IPI00007411 113 59 
Method/cell type Protein description IPI Number of hits Unique peptides 
cAMP resin/untransfected PKA type I-α R-subunit IPI00021831 64 19 
 PKA type II-α R-subunit IPI00219774 104 31 
 PKA type II-β R-subunit IPI00554752 
 AKAP9 IPI00019223 148 70 
 AKAP1 IPI00022585 
cAMP resin/stably transfected TAP-tagged wild-type RIα PKA type I-α R-subunit IPI00021831 57 19 
PKA type II-α R-subunit IPI00219774 15 12 
 PKA type II-β R-subunit IPI00554752 
 AKAP11 IPI00007411 
 AKAP9 IPI00220628 
 AKAP5 IPI00307794 
Streptavidin and calmodulin resins/stably transfected TAP-tagged wild-type RIα PKA type I-α R-subunit IPI00021831 434 29 
PKA α–catalytic subunit IPI00217960 
 PKA α–catalytic subunit IPI00396630 
 AKAP11 IPI00007411 13 
Streptavidin and cAMP resins/stably transfected TAP-tagged wild-type RIα PKA type I-α R-subunit IPI00021831 721 63 
PKA type I-β R-subunit IPI00787996 21 14 
 AKAP11 IPI00007411 113 59 

IPI, International Protein Index.

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