Acetylated microtubule rootlet length and eyespot position in wild-type, mlt1, and mlt1 uni1 cells
| Exp | Strain | na | R1/Lb | n (R2)c | R1/R2d | E1/Le | Ef | E1 at Dg | n (E2)h | E2/L | E2 at Di |
| µm | |||||||||||
| 1 | Wild type | 54 | 0.66 ± 0.11 | 50 | 1.85 ± 0.62 | ||||||
| 2 | Wild type | 51 | 0.66 ± 0.12 | 36 | 2.02 ± 0.53 | ||||||
| 3 | Wild type | 53 | 0.58 ± 0.12 | 45 | 1.78 ± 0.56 | ||||||
| 4 | Wild type | 100 | 0.75 ± 0.10 | ||||||||
| 1 | mlt1 | 36 | 0.50 ± 0.12 | 33 | 1.33 ± 0.23 | ||||||
| 2 | mlt1 | 50 | 0.38 ± 0.14 | 47 | 1.42 ± 0.50 | ||||||
| 3 | mlt1 | 50 | 0.44 ± 0.12 | 50 | 1.55 ± 0.46 | ||||||
| 4 | mlt1 | 96 | 0.63 ± 0.15 | ||||||||
| 5 | Wild type | 22 | 0.69 ± 0.09 | 0.60 ± 0.06 | 1.02 ± 0.19 | ||||||
| 6 | Wild type | 13 | 0.74 ± 0.10 | 0.65 ± 0.05 | 0.93 ± 0.14 | ||||||
| 7 | Wild type | 19 | 0.66 ± 0.11 | 0.58 ± 0.05 | 0.84 ± 0.15 | ||||||
| 8 | Wild type | 45 | 0.71 ± 0.07 | 0.61 ± 0.06 | |||||||
| 9 | Wild type | 29 | 0.74 ± 0.10 | 0.64 ± 0.06 | |||||||
| 10 | Wild type | 11 | 0.63 ± 0.06 | ||||||||
| 11 | Wild type | 16 | 0.63 ± 0.07 | ||||||||
| 12 | Wild type | 7 | 0.63 ± 0.05 | ||||||||
| 13 | Wild type | 35 | 0.67 ± 0.09 | ||||||||
| 15 | mlt1 | 27 | 0.32 ± 0.13 | 16 | 0.23 ± 0.07 | ||||||
| 16 | mlt1 | 100 | 0.36 ± 0.10 | 42 | 0.27 ± 0.07 | ||||||
| 17 | mlt1 uni1 uniflag. cellsj | 20 | 0.37 ± 0.15 | 17 | 11 | 0.19 ± 0.05 | 9 | ||||
| 18 | mlt1 uni1 uniflag. cells | 51 | 0.43 ± 0.15 | 41 | 23 | 0.24 ± 0.08 | 8 | ||||
| 18 | uni1 | 32 | 0.61 ± 0.10 | 31 |
| Exp | Strain | na | R1/Lb | n (R2)c | R1/R2d | E1/Le | Ef | E1 at Dg | n (E2)h | E2/L | E2 at Di |
| µm | |||||||||||
| 1 | Wild type | 54 | 0.66 ± 0.11 | 50 | 1.85 ± 0.62 | ||||||
| 2 | Wild type | 51 | 0.66 ± 0.12 | 36 | 2.02 ± 0.53 | ||||||
| 3 | Wild type | 53 | 0.58 ± 0.12 | 45 | 1.78 ± 0.56 | ||||||
| 4 | Wild type | 100 | 0.75 ± 0.10 | ||||||||
| 1 | mlt1 | 36 | 0.50 ± 0.12 | 33 | 1.33 ± 0.23 | ||||||
| 2 | mlt1 | 50 | 0.38 ± 0.14 | 47 | 1.42 ± 0.50 | ||||||
| 3 | mlt1 | 50 | 0.44 ± 0.12 | 50 | 1.55 ± 0.46 | ||||||
| 4 | mlt1 | 96 | 0.63 ± 0.15 | ||||||||
| 5 | Wild type | 22 | 0.69 ± 0.09 | 0.60 ± 0.06 | 1.02 ± 0.19 | ||||||
| 6 | Wild type | 13 | 0.74 ± 0.10 | 0.65 ± 0.05 | 0.93 ± 0.14 | ||||||
| 7 | Wild type | 19 | 0.66 ± 0.11 | 0.58 ± 0.05 | 0.84 ± 0.15 | ||||||
| 8 | Wild type | 45 | 0.71 ± 0.07 | 0.61 ± 0.06 | |||||||
| 9 | Wild type | 29 | 0.74 ± 0.10 | 0.64 ± 0.06 | |||||||
| 10 | Wild type | 11 | 0.63 ± 0.06 | ||||||||
| 11 | Wild type | 16 | 0.63 ± 0.07 | ||||||||
| 12 | Wild type | 7 | 0.63 ± 0.05 | ||||||||
| 13 | Wild type | 35 | 0.67 ± 0.09 | ||||||||
| 15 | mlt1 | 27 | 0.32 ± 0.13 | 16 | 0.23 ± 0.07 | ||||||
| 16 | mlt1 | 100 | 0.36 ± 0.10 | 42 | 0.27 ± 0.07 | ||||||
| 17 | mlt1 uni1 uniflag. cellsj | 20 | 0.37 ± 0.15 | 17 | 11 | 0.19 ± 0.05 | 9 | ||||
| 18 | mlt1 uni1 uniflag. cells | 51 | 0.43 ± 0.15 | 41 | 23 | 0.24 ± 0.08 | 8 | ||||
| 18 | uni1 | 32 | 0.61 ± 0.10 | 31 |
Exp, experiment number. Individual experiments were performed at separate times using unique cultures. Cells were analyzed by indirect immunofluorescence using antibodies specific for acetylated tubulin or the eyespot photoreceptor ChR1.
Number of cells analyzed.
Distance from the basal bodies to the end of the most highly acetylated rootlet (R1) relative to the anterior–posterior length of the cell (L), as diagrammed in Fig. 1 d. Each number presented is the mean of ratios obtained from individual cells and includes the SD within the experiment. R1/L values obtained from wild-type cells in experiments 1−4 (mean R1/L = 0.68 ± 0.12, n = 258) were significantly different than those obtained from mlt1 cells (mean R1/L = 0.51 ± 0.17, n = 232) with P (0.05) = 1.6 × 10
Number of cells in which the distance from the basal bodies to the second most highly acetylated rootlet (R2) was measured.
R1/R2 values obtained from wild-type cells in experiments 1–3 (mean R1/R2 = 1.87 ± 0.58, n = 131) were significantly different than those obtained from mlt1 cells (mean R1/R1 = 1.44 ± 0.44, n = 130) with P (0.05) = 1.5 × 10−10.
Distance from the basal bodies to the posterior end of the single ChR1 patch (wild-type and uni1 cells) or the most posterior ChR1 patch (mlt1 and mlt1 uni1 cells; E1) relative to the anterior–posterior length of the cell (L), as diagrammed in Fig. 1 d. Each number presented is the mean of ratios obtained from individual cells and includes the SD within the experiment. E1/L values obtained from wild-type cells in experiments 5–13 (mean E1/L = 0.61 ± 0.06, n = 128) were significantly different than those obtained from mlt1 cells in experiments 15 and 16 (mean E1/L = 0.35 ± 0.10, n = 126) with P (0.05) = 2.0 × 10−63 or from mlt1 uni1 cells in experiments 17 and 18 (0.41 ± 0.15, n = 71) with P (0.05) = 1.4 × 10−17. E1/L values obtained from uni1 cells in experiment 18 (mean E1/L = 0.61 ± 0.1, n = 34) were not significantly different than wild-type values but were significantly different than those obtained from mlt1 uni1 cells with P (0.05) = 1.1 × 10−17.
Anterior–posterior length of the ChR1 patch of the wild-type eyespot. The mean wild-type E = 0.94 ± 0.18 µm (n = 54, experiments 5–8).
Number of uni1 or mlt1 uni1 cells in which the most posterior ChR1 patch was associated with a daughter rootlet.
Number of mlt1 or mlt1 uni1 cells in which the distance from the basal bodies to the second most posterior ChR1 patch (E2) was measured.
Number of mlt1 uni1 cells in which the second most posterior ChR1 patch was associated with a daughter rootlet.
Uniflagellate mlt1 uni1 cells in which the association between ChR1 and the mother versus daughter halves of the cell was clear were chosen for analysis. Cells were from any one of six mlt1 uni1 spores.