Table II.

Yeast plasmids

Plasmid Description Marker 
pCR100 IRE1-3xFLAG, pRS306 URA3 
pCR101 ire1(D797N, K799N)-3xFLAG, pRS306 URA3 
pCR102 ire1(D828A)-3xFLAG, pRS306 URA3 
pCR103 IRE1-3xFLAG, pRS303 HIS3 
pCR104 ire1(D797N, K799N)-3xFLAG, pRS303 HIS3 
pPM56 TDH3-ero-GFP, 4xUPRE-mCherry (Merksamer et al., 2008) URA3, kanMX6 
pJK59 Sec63-GFP, CEN (Prinz et al., 2000) URA3 
Plasmid Description Marker 
pCR100 IRE1-3xFLAG, pRS306 URA3 
pCR101 ire1(D797N, K799N)-3xFLAG, pRS306 URA3 
pCR102 ire1(D828A)-3xFLAG, pRS306 URA3 
pCR103 IRE1-3xFLAG, pRS303 HIS3 
pCR104 ire1(D797N, K799N)-3xFLAG, pRS303 HIS3 
pPM56 TDH3-ero-GFP, 4xUPRE-mCherry (Merksamer et al., 2008) URA3, kanMX6 
pJK59 Sec63-GFP, CEN (Prinz et al., 2000) URA3 
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