Plasmids used in this study
| Plasmid | Description | Backbone | Source/reference |
| pCS110 | WT IRE1 gene (Trp) | pRS314 (cen/ars) | Cox et al., 1993 |
| pCS122 | WT IRE1 gene (Trp) | yEPLac112 (2µ) | Shamu and Walter, 1996 |
| pMR713 | WT KAR2 gene (Leu) | pRS315 (cen/ars) | Ng et al, 2000 |
| pMHR001 | K702A mutation in IRE1 | pCS110 | This study |
| pMHR002 | D828A mutation in IRE1 | pCS110 | This study |
| pMHR003 | S840A/S841A/T844A mutations in IRE1 | pCS110 | This study |
| pMHR004 | D828A mutation in IRE1 | pCS122 | This study |
| pMHR005 | S840D/S841D/T844D mutations in IRE1 | pCS110 | This study |
| pCF210 | Escherichia coli expression plasmid for GST-IRE1 (aa 556–1,115) | pGEX-6P-2 | Sidrauski and Walter, 1997 |
| pMHR006 | D797A mutation in Ire1 | pCS110 | This study |
| pMHR007 | R843A mutation in Ire1 | pCS110 | This study |
| pMHR008 | F842A mutation in Ire1 | pCS110 | This study |
| pMHR009 | E. coli expression plasmid for His-IRE1 (aa 642–1,115) | pET15b | This study |
| pMHR010 | D828A/S840A/S841A/T844A mutations in IRE1 | pCS110 | This study |
| pMHR011 | D828N mutation in IRE1 | pCS110 | This study |
| pMHR012 | E. coli expression plasmid for His-IRE1 (aa 642–1,111) with D828A | pMHR009 | This study |
| Plasmid | Description | Backbone | Source/reference |
| pCS110 | WT IRE1 gene (Trp) | pRS314 (cen/ars) | Cox et al., 1993 |
| pCS122 | WT IRE1 gene (Trp) | yEPLac112 (2µ) | Shamu and Walter, 1996 |
| pMR713 | WT KAR2 gene (Leu) | pRS315 (cen/ars) | Ng et al, 2000 |
| pMHR001 | K702A mutation in IRE1 | pCS110 | This study |
| pMHR002 | D828A mutation in IRE1 | pCS110 | This study |
| pMHR003 | S840A/S841A/T844A mutations in IRE1 | pCS110 | This study |
| pMHR004 | D828A mutation in IRE1 | pCS122 | This study |
| pMHR005 | S840D/S841D/T844D mutations in IRE1 | pCS110 | This study |
| pCF210 | Escherichia coli expression plasmid for GST-IRE1 (aa 556–1,115) | pGEX-6P-2 | Sidrauski and Walter, 1997 |
| pMHR006 | D797A mutation in Ire1 | pCS110 | This study |
| pMHR007 | R843A mutation in Ire1 | pCS110 | This study |
| pMHR008 | F842A mutation in Ire1 | pCS110 | This study |
| pMHR009 | E. coli expression plasmid for His-IRE1 (aa 642–1,115) | pET15b | This study |
| pMHR010 | D828A/S840A/S841A/T844A mutations in IRE1 | pCS110 | This study |
| pMHR011 | D828N mutation in IRE1 | pCS110 | This study |
| pMHR012 | E. coli expression plasmid for His-IRE1 (aa 642–1,111) with D828A | pMHR009 | This study |
cen/ars, centromeric/autonomously replicating sequence.