MT growth speeds and MT growth lifetimes in HUVECs cultured on 2D ECMs or in 3D collagen-PA-glass sandwich cultures calculated for the entire cell area (whole cell) and subcellular regions (cell branches [Branches] vs. peripheral cell body [Periphery]). ECM condition refers to collagen coupled to PA of 8.7 kPa shear modulus (2D 8.7 kPa) or a 3D collagen gel coupled to PA of a defined shear modulus with (3D 8.7 kPa + Blebb) or without treatment with 20 µM blebbistatin (3D 8.7 kPa or 3D 0.7 kPa). Mean values reported are ± standard error of the mean. n, number of MT growth tracks.