MT growth dynamics in 2D ECMs
| ECM condition | n | Mean speed | Mean lifetime |
| µm/min | s | ||
| Whole cell | |||
| Glass | 4,623 | 5.02 ± 0.254 | 9.65 ± 0.596 |
| 2D 0.7 kPa | 3,874 | 8.90 ± 0.282 | 8.71 ± 0.366 |
| Glass + Blebb | 3,235 | 9.10 ± 0.323 | 7.66 ± 0.354 |
| Branches | |||
| Glass | 18 | 5.10 ± 1.08 | 9.56 ± 1.17 |
| 2D 0.7 kPa | 173 | 8.52 ± 0.344 | 11.6 ± 0.488 |
| Glass + Blebb | 251 | 8.62 ± 0.299 | 9.82 ± 0.394 |
| Periphery | |||
| Glass | 2,285 | 4.93 ± 0.237 | 13.3 ± 0.747 |
| 2D 0.7 kPa | 1,888 | 9.55 ± 0.425 | 11.8 ± 0.667 |
| Glass + Blebb | 1,029 | 9.39 ± 0.535 | 10.5 ± 0.744 |
| ECM condition | n | Mean speed | Mean lifetime |
| µm/min | s | ||
| Whole cell | |||
| Glass | 4,623 | 5.02 ± 0.254 | 9.65 ± 0.596 |
| 2D 0.7 kPa | 3,874 | 8.90 ± 0.282 | 8.71 ± 0.366 |
| Glass + Blebb | 3,235 | 9.10 ± 0.323 | 7.66 ± 0.354 |
| Branches | |||
| Glass | 18 | 5.10 ± 1.08 | 9.56 ± 1.17 |
| 2D 0.7 kPa | 173 | 8.52 ± 0.344 | 11.6 ± 0.488 |
| Glass + Blebb | 251 | 8.62 ± 0.299 | 9.82 ± 0.394 |
| Periphery | |||
| Glass | 2,285 | 4.93 ± 0.237 | 13.3 ± 0.747 |
| 2D 0.7 kPa | 1,888 | 9.55 ± 0.425 | 11.8 ± 0.667 |
| Glass + Blebb | 1,029 | 9.39 ± 0.535 | 10.5 ± 0.744 |
MT growth speeds and MT growth lifetimes in HUVECs cultured on 2D ECMs calculated for the entire cell area (whole cell) and subcellular regions (cell branches [Branches] vs. peripheral cell body [Periphery]). ECM condition refers to collagen-coated glass with (Glass + Blebb) or without (Glass) treatment with 20 µM blebbistatin or collagen coupled to PA of 0.7 kPa shear modulus (0.7 kPa). Mean values reported are ± standard error of the mean. n, number of MT growth tracks.