Cloning details of unc45b, hsp90a, and Capza1 expression constructs
| Constructs . | Cloned with . | Amino acids . | Artificial ATG . | Utilized primers . |
|---|---|---|---|---|
| unc45-gfp | E/X | 1–934 | no | 1 + 6 |
| ΔUCS-gfp | E/X | 1–509 | no | 1 + 4 |
| ΔTPR-gfp | E/X | 120–509 | yes | 3 + 6 |
| UCS-gfp | E/X | 510–934 | yes | 5 + 6 |
| TPR-gfp | E/X | 1–119 | no | 1 + 2 |
| central-gfp | E/X | 120–509 | yes | 3 + 4 |
| Hsp90a-gfp | B/X | 1–725 | no | 7 + 8 |
| Hsp90aΔ−gfp | B/X | 1–720 | no | 7 + 9 |
| CapZa1 | E/X | 1–292 | no | 10 + 11 |
| Constructs . | Cloned with . | Amino acids . | Artificial ATG . | Utilized primers . |
|---|---|---|---|---|
| unc45-gfp | E/X | 1–934 | no | 1 + 6 |
| ΔUCS-gfp | E/X | 1–509 | no | 1 + 4 |
| ΔTPR-gfp | E/X | 120–509 | yes | 3 + 6 |
| UCS-gfp | E/X | 510–934 | yes | 5 + 6 |
| TPR-gfp | E/X | 1–119 | no | 1 + 2 |
| central-gfp | E/X | 120–509 | yes | 3 + 4 |
| Hsp90a-gfp | B/X | 1–725 | no | 7 + 8 |
| Hsp90aΔ−gfp | B/X | 1–720 | no | 7 + 9 |
| CapZa1 | E/X | 1–292 | no | 10 + 11 |
Primer 1, catcgaattcagtc atgacgatgggagaaattgg; primer 2, tagctcgagagtccagaaaggtcttgttttttggctc; primer 3, catcgaattcagtcatggagaccctcaggagacttggagctgaa; primer 4, tagctcgagagtcctcgttggaaaagggctttataag; primer 5, catcgaattcagtcatggagaccctcaggagacttggagctgaa; primer 6, tagctcgagagtcctcgttggaaaagggctttataag; primer 7, ctgggatcctcgatgcctgagaagtcggcccag; primer 8, gtaggaattcaaggtagtcgacttcctccattctc; primer 9, gtaggaattcagctctgaagtgtcgtcgtctccctc; primer 10, cgacgaattcaccgatgcagtttgacagggcaggtccag; primer 11, gagctcgagtgtgctgagcgttctgcatctctttgccg. E, EcoRI; B, BamHI; X, XhoI.